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Related Experiment Video

Updated: Jun 18, 2026

A Versatile Mounting Method for Long Term Imaging of Zebrafish Development
06:55

A Versatile Mounting Method for Long Term Imaging of Zebrafish Development

Published on: January 26, 2017

Versatile Dual Mounting Enables Larval Zebrafish Imaging Across Microscope Configurations.

Hannah Murphy1, Laura Fontenas2

  • 1Stiles-Nicholson Brain Institute Neuroscience Graduate Program, Florida Atlantic University, Jupiter, FL, USA.

Bio-Protocol
|June 17, 2026
PubMed
Summary
This summary is machine-generated.

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This new protocol allows researchers to image larval zebrafish from both dorsal and ventral views without remounting. This dual-orientation mounting method enhances flexibility and reproducibility in live imaging experiments.

Area of Science:

  • Developmental Biology
  • Microscopy Techniques

Background:

  • Larval zebrafish are conventionally mounted laterally for standardized imaging.
  • This method limits optical accessibility and requires remounting for different microscope configurations.

Purpose of the Study:

  • To present a novel mounting protocol for larval zebrafish.
  • To enable dual-orientation (dorsal and ventral) optical access without remounting.

Main Methods:

  • Utilized common laboratory consumables to create a dual-configuration mounting platform.
  • Established a hydrophobic seal for easy inversion of mounted samples.
  • Preserved lateral sample positioning in agarose.

Main Results:

  • Developed a protocol allowing seamless transitions between upright and inverted microscopes.
Keywords:
InvertedLarvaeMicroscopyMountingUprightZebrafish

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Multilayer Mounting for Long-term Light Sheet Microscopy of Zebrafish
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Multilayer Mounting for Long-term Light Sheet Microscopy of Zebrafish

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A Layered Mounting Method for Extended Time-Lapse Confocal Microscopy of Whole Zebrafish Embryos
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A Layered Mounting Method for Extended Time-Lapse Confocal Microscopy of Whole Zebrafish Embryos

Published on: January 14, 2020

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Last Updated: Jun 18, 2026

A Versatile Mounting Method for Long Term Imaging of Zebrafish Development
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A Versatile Mounting Method for Long Term Imaging of Zebrafish Development

Published on: January 26, 2017

Multilayer Mounting for Long-term Light Sheet Microscopy of Zebrafish
07:28

Multilayer Mounting for Long-term Light Sheet Microscopy of Zebrafish

Published on: February 27, 2014

A Layered Mounting Method for Extended Time-Lapse Confocal Microscopy of Whole Zebrafish Embryos
08:55

A Layered Mounting Method for Extended Time-Lapse Confocal Microscopy of Whole Zebrafish Embryos

Published on: January 14, 2020

  • Eliminated the need for specimen remounting between different microscope setups.
  • Ensured consistent anatomical positioning and experimental continuity.

    • Conclusions:

    • The protocol provides flexible, reproducible, and minimally handled live imaging of zebrafish.
    • Applicable to experiments requiring adaptable imaging geometry and high reproducibility.
    • Facilitates reliable identification and longitudinal tracking of biological regions of interest.