In-Culture Antibody Capture Using Transient CHO Expression Systems

Area of Science:

• Biotechnology
• Immunology
• Protein Engineering

Background:

• Therapeutic antibodies offer significant patient benefits, but discovery advances create production challenges.
• High-throughput screening demands rapid production of diverse antibody candidates in specific quantities.

Purpose of the Study:

• To present a semi-automated protocol for efficient, scalable antibody production.
• To bridge the gap between high-throughput screening and conventional production workflows.

Main Methods:

• Utilized a high-titer transient Chinese hamster ovary (CHO) cell expression system (CHO4Tx®).
• Employed magnetic protein-A bead in-culture antibody capturing.
• Implemented a semi-automated purification process using the GenScript AmMag™ SA Plus system.

Main Results:

• Demonstrated robust and valuable routine production of dozens of antibody constructs weekly.
• Achieved sufficient quality and quantity for cell-based and biophysical studies.
• Protocol increases production throughput by decreasing culture volume and eliminating complex steps.

Conclusions:

• The developed protocol is effective for rapid, routine production of therapeutic antibody candidates.
• Magnetic bead purification is adaptable to various cell lines, including other CHO and human embryonic kidney-293 cells.
• This workflow streamlines antibody production for research and development needs.