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Antimicrobial and antioxidant properties of green synthesis CuO nanoparticles using Salvia officinalis hydroalcoholic
Abdolrazagh Marzban1, Pegah Shakib1, Seyedeh Zahra Mirzaei2
1Razi Herbal Medicines Research Center, Lorestan University of Medical Sciences, Khorramabad, Iran.
Background And Objectives:
Following the emergence of microbial resistance to chemical antimicrobial agents, researchers have recently focused on the effects of nanoparticles and their antimicrobial activity. This research aimed to investigate the antimicrobial and antioxidant activity of copper oxide nanoparticles (CuO-NPs) produced using the hydroalcoholic extract of Salvia officinalis (S. officinalis).
Materials And Methods:
In this study, after preparing the hydroalcoholic extract of S. officinalis and synthesizing CuO-NPs using this extract, the synthesized nanoparticles were evaluated. The antimicrobial properties of synthesized CuO-NPs were investigated by determining the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) using the microbroth dilution method. The minimum biofilm inhibitory concentration (MBIC) and antioxidant activity of CuO-NPs and the hydroalcoholic extract of S. officinalis against Pseudomonas aeruginosa (P. aeruginosa) were investigated.
Results:
CuO-NPs have a size in the range of 40 nm and a monoclinic crystal structure. Antimicrobial activity assessment by well diffusion showed that the synthesized CuO-NP nanoparticles at concentrations below 5 mg/mL had no antimicrobial effect on any of the bacteria studied. The lowest MIC of the synthesized CuO-NPs was observed against Staphylococcus saprophyticus (S. saprophyticus), and the highest against P. aeruginosa and Bacillus cereus (B. cereus). On the other hand, the highest MBC was related to B. cereus. The plant extract exhibited much weaker antimicrobial activity than CuO-NPs. The results obtained showed that the MBIC for CuO-NPs was 312 μg/mL. The half-maximal inhibitory concentration (IC50) obtained from CuO-NPs and the hydroalcoholic extract of S. officinalis was 2.79 and 1.97mg/ml, respectively.
Conclusion:
Based on the results of this study, the green synthesis of CuO-NPs using hydroalcoholic extract of S. officinalis is a suitable candidate for the preparation of antimicrobial and antibiofilm compounds that also have antioxidant properties.
