Related Experiment Video
Updated: Jun 18, 2026
08:20
A Hyperandrogenic Mouse Model to Study Polycystic Ovary Syndrome
Published on: October 2, 2018
Chronic Exposure to 6PPD-Q Induces HPO Axis Dysfunction and Metabolic Disturbances in Female Offspring Mice
Yan Jiang1, Yanqi Ruan1, Zihan Zeng1
1School of Medical Technology and Information Engineering, Zhejiang Chinese Medical University, Hangzhou 310053, Zhejiang, China.
Journal of Agricultural and Food Chemistry
|June 17, 2026
Summary
N-(1,3-dimethylbutyl)-N'-phenyl-p-phenylenediamine-quinone (6PPD-Q) causes reproductive and metabolic harm in female mice by disrupting the hypothalamus-pituitary-ovary axis and hormone secretion.
Area of Science:
- Environmental Toxicology
- Reproductive Toxicology
- Endocrinology
Background:
- 6PPD-Q is an emerging environmental pollutant.
- Limited research exists on its reproductive toxicity.
Purpose of the Study:
- To investigate the reproductive toxicity and metabolic impact of 6PPD-Q exposure in female mice.
- To elucidate the mechanisms underlying 6PPD-Q's effects on the reproductive system.
Main Methods:
- Female offspring mice were exposed to 6PPD-Q during gestation and lactation.
- In vivo and in vitro experiments were conducted to assess HPO axis function, hormone secretion, and oxidative stress.
Main Results:
- 6PPD-Q exposure disrupted the hypothalamus-pituitary-ovary (HPO) axis, leading to precocious puberty and estrous cycle irregularities.
- 6PPD-Q suppressed GnRH secretion via the Kisspeptin/GPR54 pathway and inhibited LH/FSH secretion via the PKC/c-Raf/ERK1/2/IEGs pathway.
- Oxidative stress was induced in KGN cells, reducing estradiol secretion.
Conclusions:
- 6PPD-Q exhibits significant reproductive toxicity and metabolic disruption in female mice.
- The study provides novel insights into the mechanisms of 6PPD-Q's adverse effects on the reproductive system.
