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Multifiber Array-Based Photometry System for Multiregional Functional Mapping in the Mouse Brain.

Manil Bradai¹, Mirna Merkler¹, Gabriela Gil¹

¹Strathclyde Institute of Pharmacy and Biomedical Sciences, University of Strathclyde, Glasgow, UK.

The European Journal of Neuroscience

|June 17, 2026

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Researchers developed an affordable multifiber array (MFA) photometry system for mesoscopic brain mapping. This tool monitors neural signals across multiple brain regions, aiding the study of brain functions and dysfunctions.

Area of Science:

Neuroscience
Biomedical Engineering
Optical Imaging

Background:

Mesoscopic functional brain mapping is crucial for understanding brain function and dysfunction.
Current fiber photometry methods face challenges with invasiveness and scalability for accessing distributed neural circuits.

Purpose of the Study:

To present an affordable multifiber array (MFA)-based photometry system for monitoring neural signals across multiple mammalian brain regions.
To demonstrate the system's capability in investigating state-dependent neural dynamics at the mesoscopic level.

Main Methods:

Developed a custom multifiber array (MFA) using 50-μm optical fibers and standard optical components.
Monitored GABAergic population activity in awake, head-fixed mice using jGCaMP8s calcium indicator.

Keywords:

GCaMP brain state cell type fiber photometry mesoscopic functional mapping

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Multi-Fiber Photometry to Record Neural Activity in Freely-Moving Animals

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Published on: October 20, 2019

Multiple-mouse Neuroanatomical Magnetic Resonance Imaging

Multiple-mouse Neuroanatomical Magnetic Resonance Imaging

Published on: February 27, 2011

Integrated the MFA photometry system with pupillometry.

Main Results:

Successfully monitored neural signals across multiple brain regions using the MFA photometry system.
Identified state-dependent and region-specific GABAergic dynamics by combining photometry with pupillometry.
Demonstrated the system's affordability and scalability for mesoscopic brain circuit analysis.

Conclusions:

The MFA-based photometry system offers a versatile and accessible tool for mesoscopic functional brain mapping.
This system facilitates novel investigations into state-dependent neural dynamics in distributed circuits.
Publicly available resources encourage wider adoption and advancement in neuroscience research.