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Assessing the Development of Murine Plasmacytoid Dendritic Cells in Peyer's Patches Using Adoptive Transfer of Hematopoietic Progenitors

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Plasmacytoid Dendritic Cells Exhibit High Transferrin Receptor Expression Without Iron Accumulation.

Carrie Corkish¹, Simon O'Shaughnessy¹, Claire Lin¹

¹School of Biochemistry and Immunology, Trinity Biomedical Sciences, Trinity College Dublin, Dundee, Ireland.

European Journal of Immunology

|June 17, 2026

Summary

Plasmacytoid dendritic cells (pDCs) manage iron by high transferrin receptor expression and balanced flux, not accumulation. This conserved program supports their antiviral functions without limiting key immune responses.

Keywords:

CD71 TFRC dendritic cells interferons metabolism nutrient transport pDC plasmacytoid proteomics transferrin receptor

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Published on: November 4, 2016

Area of Science:

Immunology
Cell Biology
Proteomics

Background:

Plasmacytoid dendritic cells (pDCs) are crucial for antiviral immunity via type I interferon (IFN-I) production.
The proteomic and metabolic landscape of pDCs, especially their iron handling, is not fully understood.

Purpose of the Study:

To define the steady-state proteome and iron metabolism of murine and human pDCs.
To compare pDC proteomic organization with other dendritic cell subsets.
To investigate the role of iron availability in pDC antiviral functions.

Main Methods:

Deep, absolute quantitative mass spectrometry for proteomic analysis.
Proteome-wide iron mapping and functional assays.
Comparison of murine splenic pDCs with conventional dendritic cells and human pDCs.

Main Results:

pDCs exhibit a conserved proteomic architecture across species with distinct metabolic features.
High transferrin receptor (TFRC) expression drives robust iron uptake in pDCs.
Iron is preferentially allocated to iron-sulfur and heme proteins, particularly in mitochondria, with balanced import/efflux.
Iron availability does not limit TLR9-induced IFN-I or TNF production in pDCs.

Conclusions:

pDCs possess a unique iron-handling program characterized by high TFRC, balanced iron flux, and targeted iron distribution.
This program supports pDC function without iron limitation impacting antiviral cytokine production.