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Updated: Jun 19, 2026

Establishment of a High-throughput Setup for Screening Small Molecules That Modulate c-di-GMP Signaling in Pseudomonas aeruginosa
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The Role of the Mediator Complex in Modulating CjXDR1-Induced Multidrug Resistance and Virulence in Clarireedia

Huangwei Zhang1,2, Chenchen Jia1, Qifan Jin1

  • 1College of Agro-grassland Science, Nanjing Agricultural University, Nanjing 210095, China.

Phytopathology
|June 17, 2026
PubMed

Insights

The Mediator complex is crucial for the pathogenic fungus Clarireedia jacksonii. This study reveals its role in gene activation, fungicide resistance, and pathogenicity, offering potential antifungal targets.

Area of Science:

  • Molecular Biology
  • Mycology
  • Biochemistry

Background:

  • The Mediator complex regulates gene expression in pathogenic fungi.
  • Its role in Clarireedia jacksonii, a turfgrass pathogen, is uncharacterized.

Purpose of the Study:

  • To systematically analyze the Mediator complex in C. jacksonii.
  • To identify its functions in transcriptional activation, pathogenicity, and fungicide resistance.

Main Methods:

  • Systematic analysis of the Mediator complex.
  • Functional characterization of ten knockout mutants.
  • Gene expression analysis of CjXDR1-induced targets and sterol biosynthesis.

Main Results:

  • Mediator subunits play distinct roles in growth, fungicide resistance, pathogenicity, and stress responses.
  • Med1, Med31, Med15, and Cdk8 are essential for pathogenicity.
  • Tail and Kinase module subunits are linked to fungicide resistance and CjXDR1 expression.
  • Med5 deletion increased propiconazole sensitivity; Cdk8 and CycC deletions conferred resistance.
  • Mediator disruption altered CjXDR1 target gene expression and sterol biosynthesis.

Conclusions:

  • This study provides the first comprehensive insight into the Mediator complex in C. jacksonii.
  • The Mediator complex is critical for multidrug resistance in C. jacksonii.
  • Mediator subunits represent potential targets for novel antifungal strategies.
Keywords:
Clarireedia jacksoniiMediator complexfungicide resistancemultidrug resistancepathogenicitystress response

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