Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Nonsense-mediated mRNA Decay02:27

Nonsense-mediated mRNA Decay

The Upf proteins that carry out nonsense-mediated decay (NMD) are found in all eukaryotic organisms, including humans. Each protein has an individual role, but they need to work in collaboration. Upf1 is an ATP-dependent RNA helicase that unwinds the RNA helix. Because Upf1 can unwind any RNA, Upf2 and Upf3 are required to help Upf1 discriminate between nonsense and normal mRNAs.
Usually, Upf3 binds to an Exon Junction Complex (EJC) at mRNA splice sites. If a ribosome fully translates the mRNA,...
Nuclear Export of mRNA02:31

Nuclear Export of mRNA

Before mRNAs are exported to the cytoplasm, it is crucial to check each mRNA for structural and functional integrity. Eukaryotic cells use several different mechanisms, collectively known as mRNA surveillance, to look for irregularities in mRNAs. Irregular or aberrant mRNA are rapidly degraded by various enzymes. If a defective mRNA escapes the surveillance, it would be translated into a protein which would either be non-functional or not function properly. One of the primary irregularities in...
mRNA Stability and Gene Expression02:51

mRNA Stability and Gene Expression

The structure and stability of mRNA molecules regulates gene expression, as mRNAs are a key step in the pathway from gene to protein. In eukaryotes, the half-life of mRNA varies from a few minutes up to several days. mRNA stability is essential in growth and development. The absence of the proteins regulating its stability, such as tristetraprolin in mice, can cause systemic issues, including bone marrow overgrowth, inflammation, and autoimmunity.
Cis-acting Elements involved in mRNA stability
mRNA Stability and Gene Expression02:51

mRNA Stability and Gene Expression

The structure and stability of mRNA molecules regulates gene expression, as mRNAs are a key step in the pathway from gene to protein. In eukaryotes, the half-life of mRNA varies from a few minutes up to several days. mRNA stability is essential in growth and development. The absence of the proteins regulating its stability, such as tristetraprolin in mice, can cause systemic issues, including bone marrow overgrowth, inflammation, and autoimmunity.
Cis-acting Elements involved in mRNA stability
Abnormal Proliferation02:23

Abnormal Proliferation

Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the daughter...
RNA Stability01:53

RNA Stability

Intact DNA strands can be found in fossils, while scientists sometimes struggle to keep RNA intact under laboratory conditions. The structural variations between RNA and DNA underlie the differences in their stability and longevity. Because DNA is double-stranded, it is inherently more stable. The single-stranded structure of RNA is less stable but also more flexible and can form weak internal bonds. Additionally, most RNAs in the cell are relatively short, while DNA can be up to 250 million...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

SERPING1 facilitates colorectal liver metastasis by modulating epithelial-mesenchymal transition and tumor microenvironment remodeling.

Biochimica et biophysica acta. Molecular basis of disease·2026
Same author

<b>Two new species of the genus <i>Xizicus</i> Gorochov, 1993 (Orthoptera: Tettigoniidae: Meconematinae) from south China</b>.

Zootaxa·2026
Same author

<i>Schistosoma japonicum</i> Worms Alter the miRNA Expression Profile of Hepatic Stellate Cells with Potential Implications for Liver Fibrosis and Hepatocellular Carcinoma.

Tropical medicine and infectious disease·2026
Same author

Temporal and qualitative analysis of injured decomposed skin tissues using ATR-FTIR combined with chemometrics.

Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy·2026
Same author

Non-coding RNAs in schistosome reproductive maturation.

Gene·2026
Same author

Blockade of the CLCF1-CNTFR axis enhances the efficacy of GPC3 CAR-T cell therapy in hepatocellular carcinoma.

Pharmacological research·2026

Related Experiment Video

Updated: Jun 19, 2026

RNA Pull-down Procedure to Identify RNA Targets of a Long Non-coding RNA
09:36

RNA Pull-down Procedure to Identify RNA Targets of a Long Non-coding RNA

Published on: April 10, 2018

RNA-binding protein ZMAT3 protects against MASLD by regulating SEPT11 mRNA stability.

Lei Sun1, Zhiwei Huang1, Ankang Wang2

  • 1Department of General Surgery (Hepatobiliary Surgery), Department of Biliary-Pancreatic Center, The Affiliated Hospital, Southwest Medical University, Luzhou 646000, China.

Cellular Signalling
|June 17, 2026
PubMed
Summary

Zinc finger matrin-type protein 3 (ZMAT3) protects against metabolic dysfunction-associated steatotic liver disease (MASLD). ZMAT3 downregulation worsens MASLD, while its overexpression offers therapeutic potential by regulating lipid metabolism and inflammation.

Keywords:
Fat acid metabolismMASLDTherapeutic targetZMAT3mRNA stability

More Related Videos

Detection of Nuclear Blebbing and DNA Leakage in Mammalian Cells by Immunofluorescence
06:23

Detection of Nuclear Blebbing and DNA Leakage in Mammalian Cells by Immunofluorescence

Published on: January 17, 2025

Exploring Sequence Space to Identify Binding Sites for Regulatory RNA-Binding Proteins
11:34

Exploring Sequence Space to Identify Binding Sites for Regulatory RNA-Binding Proteins

Published on: August 9, 2019

Related Experiment Videos

Last Updated: Jun 19, 2026

RNA Pull-down Procedure to Identify RNA Targets of a Long Non-coding RNA
09:36

RNA Pull-down Procedure to Identify RNA Targets of a Long Non-coding RNA

Published on: April 10, 2018

Detection of Nuclear Blebbing and DNA Leakage in Mammalian Cells by Immunofluorescence
06:23

Detection of Nuclear Blebbing and DNA Leakage in Mammalian Cells by Immunofluorescence

Published on: January 17, 2025

Exploring Sequence Space to Identify Binding Sites for Regulatory RNA-Binding Proteins
11:34

Exploring Sequence Space to Identify Binding Sites for Regulatory RNA-Binding Proteins

Published on: August 9, 2019

Area of Science:

  • Hepatology
  • Molecular Biology
  • Metabolic Disorders

Background:

  • Metabolic dysfunction-associated steatotic liver disease (MASLD) is a growing hepatic disorder linked to metabolic syndrome.
  • The role of Zinc finger matrin-type protein 3 (ZMAT3), an RNA-binding protein, in MASLD pathogenesis remains unclear.

Purpose of the Study:

  • To investigate the function of ZMAT3 in MASLD.
  • To explore ZMAT3's impact on lipid metabolism and related signaling pathways in MASLD.

Main Methods:

  • Established a murine MASLD model with hepatocyte-specific ZMAT3 overexpression using a choline-deficient amino acid (CDAA) diet.
  • Utilized in vitro models with AML12 hepatocytes treated with free fatty acids to assess ZMAT3 effects.
  • Identified SEPT11 as a ZMAT3 interacting partner and employed molecular biology assays.

Main Results:

  • ZMAT3 expression was decreased in early-stage MASLD liver tissues and disease models.
  • ZMAT3 overexpression in AML12 cells reduced lipid accumulation and metabolic disturbances.
  • Hepatic ZMAT3 overexpression in MASLD mice attenuated liver injury, inflammation, and lipid accumulation.
  • ZMAT3 regulates SEPT11 mRNA stability, impacting the RhoA/ROCK1/AMPK/SREBP-1c pathway involved in MASLD.

Conclusions:

  • ZMAT3 functions as a protective factor in MASLD.
  • ZMAT3 influences lipid metabolism and the SEPT11/RhoA/ROCK1/AMPK/SREBP-1c pathway.
  • ZMAT3 presents a potential therapeutic target for MASLD.