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Related Concept Videos

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Factors Affecting Dissolution: Drug Permeability, Stability and Stereochemistry

Orally administered drugs primarily enter the systemic circulation via passive diffusion through the intestinal membranes. The drug's absorption is influenced by drug stability in the gastrointestinal GI tract, membrane permeability, the surface area available for absorption, luminal drug concentration, and residence time in the lumen. Drug permeability can be enhanced by adjusting the lipophilicity, polarity, or molecular size of the drug, promoting its passive transport across intestinal...
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Related Experiment Video

Updated: Jun 19, 2026

A Semi-Quantitative Drug Affinity Responsive Target Stability (DARTS) assay for studying Rapamycin/mTOR interaction
05:28

A Semi-Quantitative Drug Affinity Responsive Target Stability (DARTS) assay for studying Rapamycin/mTOR interaction

Published on: August 27, 2019

DORSSAA: Drug-target interactOmics Resource based on Stability/Solubility Alteration Assay.

Ehsan Zangene1, Elham Gholizadeh1, Veit Schwämmle2

  • 1Department of Biochemistry and Developmental Biology, University of Helsinki, Helsinki, Finland.

Molecular & Cellular Proteomics : MCP
|June 17, 2026
PubMed
Summary
This summary is machine-generated.

We developed DORSSAA, a web platform integrating drug-protein interaction data from stability and solubility assays. This resource aids in discovering drug targets and understanding drug mechanisms more reliably.

Related Experiment Videos

Last Updated: Jun 19, 2026

A Semi-Quantitative Drug Affinity Responsive Target Stability (DARTS) assay for studying Rapamycin/mTOR interaction
05:28

A Semi-Quantitative Drug Affinity Responsive Target Stability (DARTS) assay for studying Rapamycin/mTOR interaction

Published on: August 27, 2019

Area of Science:

  • Proteomics
  • Pharmacology
  • Bioinformatics

Background:

  • High-throughput methods like Thermal Proteome Profiling (TPP) and Proteome Integral Solubility Alteration (PISA) advance drug-protein interaction studies.
  • A unified platform for analyzing stability and solubility alteration data is lacking, hindering cross-study comparisons.

Purpose of the Study:

  • Introduce DORSSAA (Drug-target interactOmics Resource based on Stability/Solubility Alteration Assay), a web platform for analyzing drug-protein interactions.
  • Facilitate systematic analysis and visualization of proteome stability and solubility alteration assay datasets.

Main Methods:

  • Developed an interactive and expandable web-based platform, DORSSAA.
  • Integrated 1,135,985 records from 38 cell lines/organisms, 135 compounds, and 40,742 protein targets.
  • Utilized case studies for methotrexate target profiling and combinatorial therapy in leukemia.

Main Results:

  • DORSSAA enables comparative drug-protein interaction analysis and therapeutic target discovery.
  • Demonstrated utility in identifying protein-drug interactions across diverse experimental contexts.
  • Provides direct, statistically controlled, protein-level evidence of drug mechanisms.

Conclusions:

  • DORSSAA accelerates drug discovery by enabling reliable identification of drug targets and off-target effects.
  • Enhances understanding of protein behavior and facilitates the discovery of potential drug combinations.
  • Offers a superior approach compared to existing databases by providing direct mechanistic evidence.