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Related Concept Videos

MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA ends...
MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA ends...

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Related Experiment Video

Updated: Jun 19, 2026

Identifying Targets of Human microRNAs with the LightSwitch Luciferase Assay System using 3'UTR-reporter Constructs and a microRNA Mimic in Adherent Cells
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Identifying Targets of Human microRNAs with the LightSwitch Luciferase Assay System using 3'UTR-reporter Constructs and a microRNA Mimic in Adherent Cells

Published on: September 28, 2011

Spatial expression analysis and target prediction give insight into microRNA function in the model cestode

Andrés Grecco1, Natalia Macchiaroli2, Marcela Alejandra Cucher1

  • 1Departamento de Microbiología, Facultad de Medicina, Universidad de Buenos Aires, Buenos Aires, Argentina; Instituto de Investigaciones en Microbiología y Parasitología Médica (IMPaM, UBA-CONICET), Universidad de Buenos Aires, Buenos Aires, Argentina.

International Journal for Parasitology
|June 17, 2026
PubMed
Summary

This study identifies microRNAs (miRNAs) in tapeworms, revealing miR-71 plays a key role in development and infection. The findings offer potential biomarkers and therapeutic targets for cestode infections.

Keywords:
CestodeHymenolepisSpatial expressionmiR-71microRNAmicroRNA targets

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Last Updated: Jun 19, 2026

Identifying Targets of Human microRNAs with the LightSwitch Luciferase Assay System using 3'UTR-reporter Constructs and a microRNA Mimic in Adherent Cells
07:19

Identifying Targets of Human microRNAs with the LightSwitch Luciferase Assay System using 3'UTR-reporter Constructs and a microRNA Mimic in Adherent Cells

Published on: September 28, 2011

Detection of miRNA Targets in High-throughput Using the 3'LIFE Assay
12:49

Detection of miRNA Targets in High-throughput Using the 3'LIFE Assay

Published on: May 25, 2015

Genome-wide Screen for miRNA Targets Using the MISSION Target ID Library
08:40

Genome-wide Screen for miRNA Targets Using the MISSION Target ID Library

Published on: April 6, 2012

Area of Science:

  • Parasitology
  • Molecular Biology
  • Genetics

Background:

  • Hymenolepis tapeworms cause prevalent human infections (hymenolepiasis), particularly in children.
  • Tapeworms exhibit complex life cycles requiring precise genetic expression control.
  • MicroRNAs (miRNAs) are crucial regulators of gene expression during development.

Purpose of the Study:

  • To characterize miRNAs in Hymenolepis tapeworms, focusing on miR-71.
  • To identify predicted miRNA target genes in Hymenolepis microstoma and Hymenolepis nana.
  • To determine the spatial expression patterns of miRNAs in Hymenolepis microstoma.

Main Methods:

  • Bioinformatic prediction of miRNA target genes.
  • In situ hybridization for spatial miRNA expression analysis in Hymenolepis microstoma specimens.

Main Results:

  • Over 14% of total genes in H. microstoma and H. nana were identified as predicted miRNA targets.
  • miR-71 had the highest number of predicted target genes, many involved in developmental processes.
  • miR-71 showed broad expression across H. microstoma tissues, including germinative, nervous, and muscular systems, and was detected in germinative cells.
  • miR-1 exhibited restricted expression, mainly in myocytes.

Conclusions:

  • This research enhances the understanding of tapeworm miRNA biology.
  • The findings provide a foundation for future experimental studies on cestode development and infection.
  • Identified miRNAs and their targets represent potential biomarkers and therapeutic targets for cestodiasis control.