Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Single-cell and spatial transcriptomics identify SPP1 + trophoblast necroptosis as a potential contributor to late-onset preeclampsia via SPP1-CD44-mediated interaction with macrophages.

Journal of translational medicine·2026
Same author

OPRK1 insufficiency results in trophoblast dysfunction and correlates with preeclampsia pathogenesis.

Journal of reproductive immunology·2026
Same author

Spatial metabolomics and transcriptomics reveal cell type-specific dynamics in the placenta of patients with late-onset preeclampsia.

Frontiers in cell and developmental biology·2025
Same author

Development and validation of preeclampsia predictive models using key genes from bioinformatics and machine learning approaches.

Frontiers in immunology·2024
Same author

ALKBH5 modulation of ferroptosis in recurrent miscarriage: implications in cytotrophoblast dysfunction.

PeerJ·2024
Same author

CBLL1 promotes endometrial stromal cell senescence via inhibiting PTEN in recurrent spontaneous abortion.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology·2024
Same journal

scGMB: A scRNA-seq Cell Classification Method Combining GCN and Mamba.

IET systems biology·2026
Same journal

Identification of Chemokine-Related Genes Derived From T and NK Cells in the Tumour Microenvironment of Ovarian Cancer Based on scRNA-Seq.

IET systems biology·2026
Same journal

Unravelling the Mechanism of Compound Kushen Injection in Treating Cervical Cancer Through Ferroptosis Regulation: An Integrated Network Pharmacology and Molecular Docking Study.

IET systems biology·2026
Same journal

Network Pharmacology and Experimental Validation to Explore the Potential Mechanism of Salvianolic Acid B in Reversing Oxaliplatin Resistance of Colorectal Cancer Cells.

IET systems biology·2026
Same journal

Integrated Analysis Identifies an Anoikis-Related Gene Signature for Predicting Prognosis in Patients With Triple-Negative Breast Cancer.

IET systems biology·2026
Same journal

Functionalist Oncology to Model the Contextuality of Dynamics and Treatment in Acute Myeloid Leukaemia.

IET systems biology·2026
See all related articles

Related Experiment Video

Updated: Jun 19, 2026

Multiplexed Fluorescent Immunohistochemical Staining of Four Endometrial Immune Cell Types in Recurrent Miscarriage
05:16

Multiplexed Fluorescent Immunohistochemical Staining of Four Endometrial Immune Cell Types in Recurrent Miscarriage

Published on: August 4, 2021

Metabolic Reprogramming in Recurrent Spontaneous Abortion: Key Biomarkers Identification and Diagnostic Model

Fan Wu1,2, Chuanmei Qin3, Xiaowei Wei3

  • 1International Peace Maternity and Child Health Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.

IET Systems Biology
|June 17, 2026
PubMed
Summary
This summary is machine-generated.

Recurrent spontaneous abortion (RSA) is linked to metabolic changes. Key genes involved in lipid metabolism were identified as potential biomarkers for RSA diagnosis and may impact immune function.

Keywords:
diagnostic modelimmune microenvironmentmetabolic reprogrammingrecurrent spontaneous abortionsingle‐cell analysis

More Related Videos

Preparation of Frozen Non-Human Primate Fetal Islets for Combined Single Nuclei RNA-Sequencing and ATAC-Sequencing, and Bulk Metabolomics
03:39

Preparation of Frozen Non-Human Primate Fetal Islets for Combined Single Nuclei RNA-Sequencing and ATAC-Sequencing, and Bulk Metabolomics

Published on: November 8, 2024

Establishment of an Experimental Mouse Model of Endometrioma to Study its Related Infertility
08:07

Establishment of an Experimental Mouse Model of Endometrioma to Study its Related Infertility

Published on: April 5, 2024

Related Experiment Videos

Last Updated: Jun 19, 2026

Multiplexed Fluorescent Immunohistochemical Staining of Four Endometrial Immune Cell Types in Recurrent Miscarriage
05:16

Multiplexed Fluorescent Immunohistochemical Staining of Four Endometrial Immune Cell Types in Recurrent Miscarriage

Published on: August 4, 2021

Preparation of Frozen Non-Human Primate Fetal Islets for Combined Single Nuclei RNA-Sequencing and ATAC-Sequencing, and Bulk Metabolomics
03:39

Preparation of Frozen Non-Human Primate Fetal Islets for Combined Single Nuclei RNA-Sequencing and ATAC-Sequencing, and Bulk Metabolomics

Published on: November 8, 2024

Establishment of an Experimental Mouse Model of Endometrioma to Study its Related Infertility
08:07

Establishment of an Experimental Mouse Model of Endometrioma to Study its Related Infertility

Published on: April 5, 2024

Area of Science:

  • Reproductive Biology
  • Metabolomics
  • Immunology

Background:

  • Recurrent spontaneous abortion (RSA) presents complex, often unknown causes.
  • Metabolic reprogramming is increasingly recognized as a factor in RSA pathogenesis.

Purpose of the Study:

  • To investigate metabolic reprogramming in RSA.
  • To identify diagnostic biomarkers for RSA.
  • To explore immune-metabolic crosstalk in RSA.

Main Methods:

  • Integrated bioinformatics analysis of GEO datasets (GSE26787, GSE165004).
  • Weighted gene co-expression network analysis (WGCNA) and machine learning (logistic regression, SVM-RFE, LASSO) for biomarker identification.
  • In vitro experiments (PCR, Western blot, CCK-8, Transwell assays) to validate SREBF2 function.
  • Immune infiltration analysis (ssGSEA, CIBERSORT) and single-cell RNA sequencing (GSE214607).

Main Results:

  • Four key genes (SREBF2, PPARG, SQLE, UCP2) related to lipid/cholesterol metabolism were upregulated in RSA.
  • SREBF2 overexpression in endometrial stromal cells negatively affected proliferation, decidualization, and trophoblast migration.
  • Significant correlations were found between model genes and specific immune cell subsets.
  • Single-cell analysis revealed altered immune cell proportions and expression patterns, particularly in macrophages.

Conclusions:

  • Metabolic reprogramming, especially lipid metabolism, is critically involved in RSA.
  • Identified genes offer potential diagnostic biomarkers for RSA.
  • Lipid metabolism alterations may impact decidual function, trophoblast behavior, and immune tolerance in RSA, suggesting therapeutic targets.