Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: Jun 19, 2026

Germ Cell Transplantation and Testis Tissue Xenografting in Mice
10:41

Germ Cell Transplantation and Testis Tissue Xenografting in Mice

Published on: February 6, 2012

Male germ cell technologies in large animals.

Gabrielle Peckham1, Nathalia DE Lima E Martins Lara1, Ina Dobrinski1

  • 1Department of Biochemistry and Molecular Biology, Cumming School of Medicine, and Faculty of Veterinary Medicine, University of Calgary, Calgary, Alberta, T2N 1N4, Canada.

The Journal of Reproduction and Development
|June 17, 2026
PubMed
Summary

Male germ cell manipulation offers a path for heritable genetic changes in large animals using spermatogonial stem cells (SSC). Challenges in culturing, editing, and transplanting SSCs need addressing for successful genetic modification and offspring.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The role of primary cilia in the testis.

Current opinion in endocrine and metabolic research·2026
Same author

Platelet-derived growth factor signaling in porcine testicular tubular somatic cells.

Cell and tissue research·2025
Same author

Alcohol alters blood-testis barrier function in an in vitro model.

F&S science·2025
Same author

Generation of Organotypic Testicular Organoids from Rat and Pig Primary Cells in Microwell Culture.

Methods in molecular biology (Clifton, N.J.)·2025
Same author

Testicular Organoid Formation in Microwell Culture.

Methods in molecular biology (Clifton, N.J.)·2025
Same author

Optimization of spermatozoa analysis in mice: A comprehensive protocol.

Tissue & cell·2024

Area of Science:

  • Reproductive Biology
  • Biotechnology
  • Genetics

Background:

  • Spermatogonial stem cells (SSC) are crucial for lifelong sperm production and heritable genetic modification in large animals.
  • Advancements in gene editing and cell transplantation enhance the potential of germ cells for transgenerational genetic inheritance.

Purpose of the Study:

  • To review the current state of male germ cell technologies in large animals.
  • To highlight tools, methodologies, applications, limitations, and ethical considerations.
  • To outline future directions for germ cell technology in large animals.

Main Methods:

  • Review of current literature on male germ cell manipulation in large animals.
  • Analysis of gene editing and transplantation techniques.
Keywords:
Genetic improvementGenome editingGerm cell transplantationLivestock biotechnologySpermatogonial stem cells (SSC)

More Related Videos

Mouse Round Spermatid Injection
08:41

Mouse Round Spermatid Injection

Published on: January 26, 2024

A Standardized Approach for Multispecies Purification of Mammalian Male Germ Cells by Mechanical Tissue Dissociation and Flow Cytometry
08:46

A Standardized Approach for Multispecies Purification of Mammalian Male Germ Cells by Mechanical Tissue Dissociation and Flow Cytometry

Published on: July 12, 2017

Related Experiment Videos

Last Updated: Jun 19, 2026

Germ Cell Transplantation and Testis Tissue Xenografting in Mice
10:41

Germ Cell Transplantation and Testis Tissue Xenografting in Mice

Published on: February 6, 2012

Mouse Round Spermatid Injection
08:41

Mouse Round Spermatid Injection

Published on: January 26, 2024

A Standardized Approach for Multispecies Purification of Mammalian Male Germ Cells by Mechanical Tissue Dissociation and Flow Cytometry
08:46

A Standardized Approach for Multispecies Purification of Mammalian Male Germ Cells by Mechanical Tissue Dissociation and Flow Cytometry

Published on: July 12, 2017

  • Discussion of challenges and ethical considerations.

    • Main Results:

    • Significant opportunities exist for heritable genetic modification via male germ cells, particularly SSCs.
    • Progress in gene editing and transplantation has advanced germ cell technology.
    • Key challenges include lack of robust SSC culture, species-specific differences, editing efficiency, and immune rejection.

    Conclusions:

    • Male germ cell technologies hold promise for genetic modification in large animals.
    • Overcoming technical and ethical hurdles is essential for clinical translation.
    • This field integrates reproductive biology, biotechnology, and translational science.