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DNA Damage Can Stall the Cell Cycle

DNA Damage Can Stall the Cell Cycle

In response to DNA damage, cells can pause the cell cycle to assess and repair the breaks. However, the cell must check the DNA at certain critical stages during the cell cycle. If the cell cycle pauses before DNA replication, the cells will contain twice the amount of DNA. On the other hand, if cells arrest after DNA replication but before mitosis, they will contain four times the normal amount of DNA. With a host of specialized proteins at their disposal,cells must use the right protein at...

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Related Experiment Video

Updated: Jun 19, 2026

Assessing Cell Cycle Progression of Neural Stem and Progenitor Cells in the Mouse Developing Brain after Genotoxic Stress

Assessing Cell Cycle Progression of Neural Stem and Progenitor Cells in the Mouse Developing Brain after Genotoxic Stress

Published on: May 7, 2014

Confined migration induces non-lethal DNA damage in developing neurons.

Zhejing Zhang^1,2, Andres Canela^2,3, Junko Kurisu¹

¹Institute for Integrated Cell-Material Science (WPI-iCeMS), Kyoto University, Kyoto, Japan.

|June 17, 2026

Summary

This summary is machine-generated.

During brain development, migrating neurons experience DNA double-stranded breaks (DSBs) from physical stress. These breaks are repaired without cell death, but unrepaired damage may pose future disease risks.

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Published on: July 25, 2017

Area of Science:

Neuroscience
Molecular Biology
Genetics

Background:

Migratory cells, including cancer cells, can experience nuclear deformation and DNA damage during migration through confined spaces.
The role and consequences of DNA damage during physiological cell migration, particularly in the developing brain, remain less understood.

Purpose of the Study:

To investigate the occurrence and mechanisms of DNA damage in migrating neurons during brain development.
To understand the cellular response and long-term implications of DNA damage in developing neurons.

Main Methods:

Utilized mouse models of brain development.
Analyzed DNA double-stranded breaks (DSBs) using molecular assays.
Performed genome sequencing to identify DSB locations.
Investigated the role of specific genes (e.g., ligase IV) in DNA repair during neuronal migration.

Main Results:

Neuronal migration in the developing cortex is associated with massive DNA double-stranded breaks (DSBs) caused by mechanical stress in narrow spaces.
These DSBs occur without nuclear envelope rupture and are repaired via non-homologous end-joining, with no observed cell death.
DSBs preferentially occur in transcriptionally inactive genomic regions.
Deletion of ligase IV leads to persistent DSBs and mild motor deficits in adult mice, indicating potential disease risk.

Conclusions:

Mechanical stress during neuronal migration in the developing brain induces significant DNA double-stranded breaks.
Efficient DNA repair mechanisms are crucial for preventing long-term consequences of developmental DNA damage.
Failure to repair these endogenous DNA breaks may contribute to neurological disorders later in life.