Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Fibril-associated Collagen01:11

Fibril-associated Collagen

Fibril-associated collagens are a type of collagens present in the extracellular matrix with interrupted triple helices or FACIT (Fibril-associated collagens interrupted triple-helices). FACIT help connect and attach the collagen fibrils with each other as well as with other proteins of the extracellular matrix.
For example, the type II collagen fibrils in cartilage have covalently bound type IX fibril-associated collagens at regular intervals. Other types of fibril-associated collagens are...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

On-glass Interference Confocal Reflectance Microscopy for Collagen Fibril Diameter Measurement.

Proceedings of SPIE--the International Society for Optical Engineering·2026
Same author

Interference Confocal Reflectance Microscopy for Collagen Fibril Diameter Measurement.

Proceedings of SPIE--the International Society for Optical Engineering·2026
Same author

Endothelial cell stiffness and type drive the formation of biomechanically induced transcellular pores.

Cell reports·2025
Same author

Transient receptor potential vanilloid 4 modulates substrate stiffness mechanosensing and transcellular pore formation in human Schlemm's canal cells.

Acta biomaterialia·2025
Same author

Matrix stiffness regulates traction forces, cytoskeletal dynamics, and collagen reorganization in trabecular meshwork cells in glaucoma.

Matter·2025
Same author

Magnetically steered cell therapy for reduction of intraocular pressure as a treatment strategy for open-angle glaucoma.

eLife·2025
Same journal

Generalizable framework for multi-site bone density prediction using non-dominant wrist optical biomarkers.

Biomedical optics express·2026
Same journal

Erratum: Review of dynamic optical coherence tomography for intracellular motility [Invited]: errata.

Biomedical optics express·2026
Same journal

Digital-micromirror-device-based illumination strategies for background suppression in single-molecule localization microscopy.

Biomedical optics express·2026
Same journal

Synergistic combination of convective self-assembly and hollow core fiber for sensitive SERS detection of glucose molecules.

Biomedical optics express·2026
Same journal

Multimodal diagnostic network integrating infrared and mass spectra for lung cancer.

Biomedical optics express·2026
Same journal

Multimodal Optical Biosensing for Precision Medicine and Healthcare: Introduction to the feature issue.

Biomedical optics express·2026
See all related articles

Related Experiment Video

Updated: Jun 19, 2026

Preparing Porcine Eyes for Confocal Reflectance Microscopy to Visualize the Vitreous Collagen Fiber Network
06:07

Preparing Porcine Eyes for Confocal Reflectance Microscopy to Visualize the Vitreous Collagen Fiber Network

Published on: October 17, 2025

Collagen fibril diameter quantification using interference confocal reflectance microscopy.

Eric Hall1, Seyed Mohammad Siadat2, Jeffrey Ruberti2

  • 1Northeastern University, Electrical and Computer Engineering, 360 Huntington Avenue, Boston, Massachusetts, USA.

Biomedical Optics Express
|June 18, 2026
PubMed
Summary
This summary is machine-generated.

This study introduces a new method using interference confocal reflectance microscopy (I-CRM) to measure collagen fibril diameters below the diffraction limit. This technique allows real-time analysis of collagen assembly and remodeling under mechanical stress.

More Related Videos

In vitro Synthesis of Native, Fibrous Long Spacing and Segmental Long Spacing Collagen
07:54

In vitro Synthesis of Native, Fibrous Long Spacing and Segmental Long Spacing Collagen

Published on: September 20, 2012

Mammalian Cell Division in 3D Matrices via Quantitative Confocal Reflection Microscopy
10:22

Mammalian Cell Division in 3D Matrices via Quantitative Confocal Reflection Microscopy

Published on: November 29, 2017

Related Experiment Videos

Last Updated: Jun 19, 2026

Preparing Porcine Eyes for Confocal Reflectance Microscopy to Visualize the Vitreous Collagen Fiber Network
06:07

Preparing Porcine Eyes for Confocal Reflectance Microscopy to Visualize the Vitreous Collagen Fiber Network

Published on: October 17, 2025

In vitro Synthesis of Native, Fibrous Long Spacing and Segmental Long Spacing Collagen
07:54

In vitro Synthesis of Native, Fibrous Long Spacing and Segmental Long Spacing Collagen

Published on: September 20, 2012

Mammalian Cell Division in 3D Matrices via Quantitative Confocal Reflection Microscopy
10:22

Mammalian Cell Division in 3D Matrices via Quantitative Confocal Reflection Microscopy

Published on: November 29, 2017

Area of Science:

  • Biophysics
  • Materials Science
  • Cell Biology

Background:

  • Understanding collagen fibril assembly and remodeling is crucial for tissue engineering and disease research.
  • Traditional microscopy methods are limited by the Abbe diffraction limit, hindering nanoscale measurements of collagen fibrils.
  • Quantifying fibril mechanics during monomer interaction is essential for understanding self-assembly and repair processes.

Purpose of the Study:

  • To develop a novel method for measuring collagen fibril diameters below the diffraction limit using confocal reflectance microscopy.
  • To integrate this method with confocal fluorescence systems for real-time tracking of collagen monomers and fibrils.
  • To enable quantification of collagen monomer interactions with fibrils under cyclic loading for insights into growth and repair.

Main Methods:

  • Utilized 2D finite difference time domain (FDTD) simulations to predict interference patterns.
  • Employed interference confocal reflectance microscopy (I-CRM) with wavelengths of 488 nm, 514 nm, and 561 nm on Type 1 collagen fibrils.
  • Correlated I-CRM results with scanning electron microscopy (SEM) images for algorithm development and validation.

Main Results:

  • The developed I-CRM method successfully measured collagen fibril diameters below the diffraction limit.
  • Interference patterns observed in I-CRM experiments matched FDTD simulation predictions.
  • An algorithm integrating FDTD and I-CRM data accurately estimated fibril diameters and allowed direct overlay with SEM images.

Conclusions:

  • Interference confocal reflectance microscopy (I-CRM) is a promising technique for measuring sub-diffraction limit collagen fibril diameters.
  • I-CRM offers advantages such as non-lethality, no need for fluorescent staining, and no-registration calibration.
  • This method facilitates real-time, quantitative analysis of collagen fibril dynamics and interactions, crucial for understanding tissue development and repair.