Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: Jun 19, 2026

Establishing a Physiologic Human Vascularized Micro-Tumor Model for Cancer Research
07:26

Establishing a Physiologic Human Vascularized Micro-Tumor Model for Cancer Research

Published on: September 15, 2023

Development of a novel protocol for processing fluorescent microspheres used in quantifying tissue perfusion.

Michael B Nelappana1,2,3, Catherine C Applegate1,2, Leopold J B Pinot2

  • 1Department of Bioengineering, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA.

Sensing and Bio-Sensing Research
|June 18, 2026
PubMed
Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Exercise Training Stimulates the Release of Glutathione Peroxidase 1 (GPX1)-Enriched Extracellular Vesicles That Promote Angiogenesis.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology·2026
Same author

Red-complex bacteria: immunological background leading to the development of head and neck cancers.

Frontiers in immunology·2026
Same author

Beyond fluorodeoxyglucose: High-definition macrophage imaging to predict heart failure.

Journal of nuclear cardiology : official publication of the American Society of Nuclear Cardiology·2026
Same author

The effect of cold ischemia time on hypoxia, EMT, and apoptosis pathways in normal colon mucosa.

PloS one·2026
Same author

Ipilimumab, -omics, and head and neck cancers-update in 2025.

Frontiers in immunology·2026
Same author

The Role of the Receptor for Advanced Glycation End-Products in Cancer: Evidence from a Systematic Review and Meta-Analysis.

bioRxiv : the preprint server for biology·2026

This study introduces a cost-efficient method for measuring blood perfusion using direct imaging of fluorescent microspheres (MS). The new protocol improves accuracy and reduces costs compared to the standard method.

Area of Science:

  • Biomedical Engineering
  • Cardiovascular Research
  • Analytical Chemistry

Background:

  • Alterations in blood perfusion are central to cardiovascular diseases.
  • Current methods using fluorescent microspheres (MS) for perfusion measurement are costly and prone to error, often underestimating regional perfusion.
  • Existing techniques risk microsphere loss and utilize hazardous chemicals.

Purpose of the Study:

  • To develop an improved, cost-efficient protocol for measuring regional blood perfusion.
  • To enable accurate quantification of fluorescent microspheres (MS) via direct ex vivo imaging.
  • To overcome limitations of current standard methods in perfusion assessment.

Main Methods:

  • Developed a novel protocol involving chemical digestion and direct imaging of fluorescent microspheres (MS) ex vivo.
Keywords:
Fluorescent imagingFluorometryMicrospheresPeripheral arterial diseaseTissue perfusion

More Related Videos

The Use of Ex Vivo Whole-organ Imaging and Quantitative Tissue Histology to Determine the Bio-distribution of Fluorescently Labeled Molecules
08:07

The Use of Ex Vivo Whole-organ Imaging and Quantitative Tissue Histology to Determine the Bio-distribution of Fluorescently Labeled Molecules

Published on: December 24, 2016

Related Experiment Videos

Last Updated: Jun 19, 2026

Establishing a Physiologic Human Vascularized Micro-Tumor Model for Cancer Research
07:26

Establishing a Physiologic Human Vascularized Micro-Tumor Model for Cancer Research

Published on: September 15, 2023

The Use of Ex Vivo Whole-organ Imaging and Quantitative Tissue Histology to Determine the Bio-distribution of Fluorescently Labeled Molecules
08:07

The Use of Ex Vivo Whole-organ Imaging and Quantitative Tissue Histology to Determine the Bio-distribution of Fluorescently Labeled Molecules

Published on: December 24, 2016

  • Compared a direct imaging method using cellulose filters with the standard fluorometric method using polycarbonate (PCTE) filters.
  • Analyzed porcine and control tissue samples treated with MS.

    • Main Results:

    • The direct imaging method with cellulose filters demonstrated significantly improved sensitivity and a lower limit of detection for MS.
    • Observed an 11-13x amplification in sample fluorescence compared to PCTE filters, with parity between porcine and control samples.
    • Achieved a 28-51% cost reduction and eliminated the need for the teratogen 2-ethoxy ethyl acetate.

    Conclusions:

    • The proposed direct imaging protocol offers a more sensitive, cost-effective, and safer alternative for measuring regional perfusion using fluorescent microspheres (MS).
    • This method enhances accuracy by avoiding filter interactions and reducing microsphere loss.
    • The improved workflow simplifies perfusion measurement in cardiovascular research and clinical applications.