Genome Sequence and Host Range Investigation of Cluster CV Phage Spink

Bacteriophage Spink was isolated using host bacterium Gordonia terrae 3612. Spink's host range was investigated across six other bacterial strains; however, its host range appeared restricted to G. terrae , as all expansion host pairings resulted in killing from without (KFW) or no infection (NI). Spink displays a siphovirus morphotype and possesses a 49,479 bp genome. Based on gene content similarity, Spink is assigned to cluster CV. Putative functions were assigned to 39 of 78 genes. Like most CV phages, Spink is temperate and encodes several host interaction genes, including a tyrosine integrase, immunity repressor, and a RelE-RelB toxin-antitoxin pair.