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Related Concept Videos

Mass Spectrometry: Complex Analysis01:21

Mass Spectrometry: Complex Analysis

Mass spectrometry is an important technique for the identification of pure compounds. However, it has some limitations for the analysis of complex mixtures, often due to excessive fragmentation making the spectrum too complicated to decipher. Mass spectrometry can be combined with suitable separation methods in sequence, forming hyphenated methods, which are useful in the analysis of complex mixtures.
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...
High-Resolution Mass Spectrometry (HRMS)01:15

High-Resolution Mass Spectrometry (HRMS)

The resolution of a mass spectrometer depends on the efficiency of separating ions with different ion masses. The mass of an atom is approximated to the sum of the masses of protons and neutrons inside, considering the masses of protons and neutrons as equal. However, the masses of the proton (1.6726 × 10−24 g) and neutron (1.6749 × 10−24 g) are not truly equal. There is a minor error in the expression of atomic masses relative to the simplest atom of hydrogen. For example, the mass of helium...
Tandem Mass Spectrometry01:21

Tandem Mass Spectrometry

Tandem mass spectrometry is a technique that uses multiple mass analyzers in series to obtain a higher selectivity and reduce chemical noise during analyte detection. Instruments with multiple analyzers separated by an interaction cell enable secondary fragmentation and selected study of the fragment ions.Secondary fragmentations occur in the interaction cell and can be induced by various factors. Fragmentation induced by collision with inert gases, such as N2, Ar, He, etc., is called...
Peptide Identification Using Tandem Mass Spectrometry01:33

Peptide Identification Using Tandem Mass Spectrometry

Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...
Chemical Ionization (CI) Mass Spectrometry01:21

Chemical Ionization (CI) Mass Spectrometry

The molecular ion peak of a molecule in the mass spectrum provides vital information for molecular identification. However, conventional electron impact ionization can lead to the rapid dissociation of some molecular ions before they reach the detector. A milder ionization method is required to increase the lifetime of such ionized analyte molecules. Chemical ionization (CI) is a gas-phase protonation reaction useful for mass-analyzing analyte molecules that are easily protonated to yield the...
Mass Spectrometry: Overview01:19

Mass Spectrometry: Overview

Mass spectrometry is an analytical technique used to determine the molecular mass and molecular formula of a compound. The basic principle of mass spectrometry is to generate ions from the analyte molecule and measure these ion abundances against their molecular mass. One common type of ionization, known as electron ionization or EI, bombards the analyte molecules in the gas phase with high-energy electron beams. The electron beams displace an electron from the molecule and leave behind a...

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Related Experiment Video

Updated: Jun 19, 2026

Sample Preparation for Single Cell Mass Spectrometry Metabolomics Studies: Combined Cell Washing, Quenching, Drying, and Storage
08:07

Sample Preparation for Single Cell Mass Spectrometry Metabolomics Studies: Combined Cell Washing, Quenching, Drying, and Storage

Published on: September 16, 2025

Coaxial Hybrid Ionization-Mass Cytometry for High-Throughput and High-Coverage Single-Cell Metabolomics.

Yi Zhang1,2, Mingxuan Li2, Qian Li1,2

  • 1Beijing Life Science Academy, Beijing 102209, China.

Analytical Chemistry
|June 18, 2026
PubMed
Summary
This summary is machine-generated.

A new Hybrid-CytoMS platform enhances single-cell metabolomics by combining two ionization methods, enabling detection of more metabolites and revealing cellular responses to environmental toxins.

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A Strategy for Sensitive, Large Scale Quantitative Metabolomics
14:18

A Strategy for Sensitive, Large Scale Quantitative Metabolomics

Published on: May 27, 2014

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Last Updated: Jun 19, 2026

Sample Preparation for Single Cell Mass Spectrometry Metabolomics Studies: Combined Cell Washing, Quenching, Drying, and Storage
08:07

Sample Preparation for Single Cell Mass Spectrometry Metabolomics Studies: Combined Cell Washing, Quenching, Drying, and Storage

Published on: September 16, 2025

A Strategy for Sensitive, Large Scale Quantitative Metabolomics
14:18

A Strategy for Sensitive, Large Scale Quantitative Metabolomics

Published on: May 27, 2014

Area of Science:

  • Analytical Chemistry
  • Biochemistry
  • Cell Biology

Background:

  • Single-cell metabolomics is crucial for understanding cellular heterogeneity.
  • Limited metabolite coverage in current methods restricts broader applications due to low abundance and diverse physicochemical properties of metabolites.

Purpose of the Study:

  • To develop an advanced platform for enhanced single-cell metabolomic analysis.
  • To improve metabolite coverage and sensitivity in mass cytometry.

Main Methods:

  • Development of a coaxial hybrid ionization-mass cytometry platform (Hybrid-CytoMS).
  • Integration of dielectric barrier discharge ionization (DBDI) with electrospray ionization (ESI).
  • High-throughput analysis at 30 cells per minute.

Main Results:

  • Hybrid-CytoMS significantly enhanced ionization efficiency and detected nonpolar compounds previously undetectable.
  • Analysis of HepG2 cells revealed >2500 features, >500 characteristic peaks, and 369 annotated metabolites.
  • The platform successfully resolved benzo[a]pyrene uptake, clearance, and metabolic trajectories in response to exposure.

Conclusions:

  • Hybrid-CytoMS offers superior performance over conventional ESI-based mass cytometry for single-cell metabolomics.
  • The platform enables deeper insights into cellular metabolic responses to exogenous compounds at single-cell resolution.
  • This technology advances the study of cellular metabolism and toxicology.