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Related Experiment Video

Updated: Jun 19, 2026

Microfabricated Platforms for Mechanically Dynamic Cell Culture
15:21

Microfabricated Platforms for Mechanically Dynamic Cell Culture

Published on: December 26, 2010

3D-Printed Platforms for Enzyme Immobilisation Screening and Direct Translation into Continuous-Flow Biocatalysis.

Simone Marchetti1, Gianluca Palmara1, Cristopher Tinajero1

  • 1Institute of Advanced Materials (INAM), Universitat Jaume I, Castellón de la Plana, Spain.

Chemsuschem
|June 18, 2026
PubMed
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This study presents a 3D-printing method for rapidly screening and developing immobilized biocatalysts. The optimized enzyme immobilization was directly applied to a flow reactor, achieving high conversion and stability for continuous chemical synthesis.

Area of Science:

  • Biotechnology
  • Chemical Engineering
  • Materials Science

Background:

  • Developing immobilized biocatalysts for continuous flow processes often relies on inefficient trial-and-error screening.
  • Optimized conditions on conventional carriers are frequently not transferable to structured reactors.

Purpose of the Study:

  • To introduce an integrated 3D-printing methodology for high-throughput screening of enzyme immobilization.
  • To enable direct implementation of optimized biocatalysts in continuous-flow reactors.

Main Methods:

  • A 3D-printing technique was used to create enzyme supports with modified surfaces using imidazolium-based supported ionic liquid phases.
  • High-throughput screening in a 96-well format evaluated alcohol dehydrogenase (ADH-200) immobilization conditions using a colorimetric assay.
Keywords:
additive manufacturingadvanced materialsbiocatalysiscontinuous‐flow reactorsenzyme immobilisationhigh‐throughput screening

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  • The best-performing formulation was applied to a 3D-printed honeycomb-structured reactor for continuous flow catalysis.

    • Main Results:

    • Methyl-imidazolium-modified supports demonstrated superior enzymatic activity and immobilization efficiency compared to other ionic liquids.
    • The functionalized 3D-printed reactor efficiently catalyzed the oxidation of 1-phenylethanol to acetophenone.
    • High conversion rates were achieved at moderate residence times with sustained biocatalyst performance over several hundred hours.

    Conclusions:

    • The integrated 3D-printed platform accelerates the identification of effective enzyme immobilization chemistries.
    • This approach facilitates the translation of optimized biocatalysts into robust and efficient flow reactors.
    • The methodology provides a general strategy for the rapid development of immobilized biocatalysts for continuous processes.