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Hesperidin Methyl Chalcone Inhibits Hallmarks of Cancer by Targeting the AKT, PI3K/GSK-3β/β-Catenin/STAT3 Signaling

Saranya Jawaharlal1, Ramachandhiran Duraisamy1, Vinothkumar Veerasamy1

  • 1Department of Biochemistry and Biotechnology, Faculty of Science, Annamalai University, Annamalainagar, Tamilnadu, India.

Journal of Biochemical and Molecular Toxicology
|June 18, 2026
PubMed

Insights

Hesperidin Methyl Chalcone (HMC) shows promise in preventing oral cancer by improving histological changes and protecting key proteins. This natural compound offers potential chemopreventive effects against oral squamous cell carcinoma.

Area of Science:

  • Oncology
  • Pharmacology
  • Biochemistry

Background:

  • Oral squamous cell carcinoma (OSCC) is a common and drug-resistant cancer.
  • DMBA-induced hamster buccal pouch carcinogenesis (HBPCs) is a model for studying oral cancer.
  • Hesperidin Methyl Chalcone (HMC) is a flavonoid derivative with potential therapeutic properties.

Purpose of the Study:

  • To investigate the chemopreventive effects of HMC on DMBA-induced HBPCs.
  • To evaluate the biochemical and histological changes in oral carcinogenesis treated with HMC.
  • To identify potential protein targets of HMC using in silico analysis.

Main Methods:

  • Induction of oral tumors in hamsters using 7,12-dimethylbenz[a]anthracene (DMBA).
  • Topical application of HMC to DMBA-painted hamster buccal pouches.
  • Biochemical and histological analysis of tumor tissues.
  • In silico molecular docking to predict protein targets.

Main Results:

  • HMC treatment significantly improved biochemical and histological alterations in DMBA-induced oral tumors.
  • In silico analysis revealed high binding affinity of HMC to Akt, Pi3k, GSk-3β, β-catenin, and STAT3.
  • These proteins showed considerable changes during DMBA-induced oral carcinogenesis.

Conclusions:

  • HMC exhibits significant chemopreventive properties against oral carcinogenesis.
  • HMC protects key proteins (Akt, Pi3k, GSk-3β, β-catenin, STAT3) involved in oral cancer development.
  • HMC is a potential therapeutic agent for preventing oral squamous cell carcinoma.
Keywords:
biochemicaldysplasiahyperplasiain silicooral carcinogenesis

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