Related Experiment Video
Updated: Jun 19, 2026
11:22
Microbiota Analysis Using Two-step PCR and Next-generation 16S rRNA Gene Sequencing
Published on: October 15, 2019
Characterisation of the Novel HLA-DRB4*01:229 Allele in a Korean Individual by Next-Generation Sequencing
Jae Hee Lee1, You Hyun Jeon2, Dong Il Won3
1Department of Laboratory Medicine, Kyungpook National University Chilgok Hospital, Daegu, Republic of Korea.
HLA
|June 18, 2026
Abstract:
HLA-DRB4*01:229 differs from HLA-DRB4*01:03:01:17 by one nucleotide substitution in codon 156 of exon 3.
Related Concept Videos
01:05Single Nucleotide Polymorphisms-SNPs
A single nucleotide polymorphism or SNP is a single nucleotide variation at a specific genomic position in a large population. It is the most prevalent type of sequence variation found in the human genome. Point mutations that occur in more than 1% of the population qualify as SNPs. These are present once every 1000 nucleotides on an average in the human genome. Replacement of a purine with another purine (A/G) or a pyrimidine with another pyrimidine (C/T) is known as a transition. In contrast,...
03:00Next-generation Sequencing
The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features.