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Related Experiment Video

Updated: Jun 20, 2026

An Integrated Workflow to Study the Promoter-Centric Spatio-Temporal Genome Architecture in Scarce Cell Populations

An Integrated Workflow to Study the Promoter-Centric Spatio-Temporal Genome Architecture in Scarce Cell Populations

Published on: April 21, 2023

CODEC Library Preparation From Genomic DNA.

James Phie¹, Joshua N Johnstone¹, Cameron Fraser¹

¹Systematic Medicine, Melbourne, Australia.

Current Protocols

|June 18, 2026

Summary

This summary is machine-generated.

This study details a validated protocol for Concatenating Original Duplex for Error Correction (CODEC) library preparation. This method enhances DNA sequencing accuracy for detecting low-frequency somatic variants.

Keywords:

CODEC NanoSeq duplex somatic variants

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Generation of Genome-wide Chromatin Conformation Capture Libraries from Tightly Staged Early Drosophila Embryos

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Published on: October 3, 2018

Area of Science:

Molecular Biology
Genomics
Biotechnology

Background:

Duplex sequencing offers enhanced accuracy by linking complementary DNA strands.
Concatenating Original Duplex for Error Correction (CODEC) is a cost-effective duplex sequencing method.
Widespread adoption of CODEC is hindered by a lack of detailed wet-lab protocols.

Purpose of the Study:

To provide a detailed, experimentally validated protocol for CODEC library preparation.
To ensure compatibility with standard Illumina sequencing platforms.
To facilitate reproducible implementation of CODEC for research applications.

Main Methods:

The protocol includes blunt-end fragmentation, dideoxy base-blocked A-tailing, and CODEC adapter ligation.
Strand-displacing extension and library amplification are incorporated.
Quality control checkpoints, critical parameters, and troubleshooting guidance are provided.

Main Results:

An experimentally validated protocol for CODEC library preparation from genomic DNA is described.
The protocol is compatible with standard Illumina sequencing platforms.
An optional matched germline reference library preparation protocol is included.

Conclusions:

This workflow enables reproducible CODEC library preparation in molecular biology labs.
It supports accurate detection of low-frequency somatic variants.
The protocol aims to broaden the adoption of cost-effective duplex sequencing.