Related Experiment Video
Updated: Jun 20, 2026

10:39
A Label-Free Segmentation Approach for Intravital Imaging of Mammary Tumor Microenvironment
Published on: May 24, 2022
SAMJ: fast image annotation on ImageJ/Fiji via segment anything model
Carlos García-López-de-Haro1,2, Caterina Fuster-Barceló3,4, Curtis T Rueden5
1Neuroscience and Life Sciences Department, Universidad Carlos III de Madrid, Getafe, Spain.
Nature Communications
|June 18, 2026
Summary
This study introduces SAMJ, a user-friendly ImageJ/Fiji plugin for fast, accurate biomedical image annotation using artificial intelligence (AI). SAMJ simplifies the creation of AI training datasets, making advanced tools accessible to researchers.
Area of Science:
- Biomedical image analysis
- Artificial intelligence in medicine
- Bioimage informatics
Background:
- Accurate AI training for biomedical image analysis requires time-consuming pixel-level annotations.
- Existing foundation models like Segment Anything Model (SAM) have limited integration into bioimage analysis platforms.
- Technical expertise and specialized hardware are often needed for advanced AI annotation.
Purpose of the Study:
- To develop a user-friendly plugin for ImageJ/Fiji that simplifies AI-assisted image annotation.
- To enable fast, interactive, and accurate annotation of biomedical images without programming skills.
- To broaden access to advanced AI annotation tools for the research community.
Main Methods:
- Integration of efficient Segment Anything Model (SAM) variants into ImageJ/Fiji via the SAMJ plugin.
- Development of a graphical user interface for interactive object segmentation using clicks or bounding boxes.
- Implementation on standard computers without requiring specialized hardware.
Main Results:
- SAMJ enables real-time delineation of objects in large scientific images.
- The plugin significantly reduces the effort and time required for image annotation.
- SAMJ facilitates faster creation of datasets for AI model training.
Conclusions:
- SAMJ provides a fast, interactive, and accurate solution for biomedical image annotation.
- The plugin lowers the barrier to entry for using AI-assisted annotation tools in research.
- SAMJ accelerates AI development in biomedical image analysis by simplifying dataset creation.
