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Related Concept Videos

Labeling DNA Probes03:31

Labeling DNA Probes

DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...

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Fluorescence Microscopy for ATP Internalization Mediated by Macropinocytosis in Human Tumor Cells and Tumor-xenografted Mice
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Fluorescence Microscopy for ATP Internalization Mediated by Macropinocytosis in Human Tumor Cells and Tumor-xenografted Mice

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Biotinylated ATP-Proton AND-Logic Fluorescent Probe toward Precision Tumor Imaging.

Yun Jae Yang1, Shayeri Biswas1,2, Sourav Sarkar1,3

  • 1Department of Chemistry, Pohang University of Science and Technology, Pohang 37673, Republic of Korea.

Analytical Chemistry
|June 19, 2026
PubMed
Summary

A novel ATP probe detects cancer by targeting biotin receptors and activating in acidic tumor environments. This highly selective probe enables rapid, precise tumor imaging in vitro and in vivo.

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Fluorescence Microscopy for ATP Internalization Mediated by Macropinocytosis in Human Tumor Cells and Tumor-xenografted Mice
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Published on: July 17, 2012

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Medical Imaging

Background:

  • Adenosine triphosphate (ATP) is crucial for cancer cell proliferation and is abundant in tumor extracellular spaces, making it a potential biomarker.
  • Existing tumor detection methods require improvement in sensitivity and specificity.

Purpose of the Study:

  • To develop and evaluate a novel ATP probe for precise tumor detection and imaging.
  • To assess the probe's selectivity and signal enhancement in various experimental settings.

Main Methods:

  • A new ATP probe was designed to target biotin receptors and respond to the acidic tumor microenvironment.
  • The probe's performance was tested using mixed-cell experiments and two-photon microscopy.
  • In vivo imaging was conducted on mice with receptor-positive tumors.

Main Results:

  • The probe demonstrated high selectivity, with a 17-fold signal enhancement in mixed-cell experiments.
  • Two-photon microscopy revealed a 13.6-fold signal enhancement in tumor tissue over normal tissue.
  • In vivo imaging showed a 7.7-fold signal enhancement distinguishing receptor-positive tumors from normal tissue.

Conclusions:

  • The multitargeting ATP probe offers a promising approach for precision tumor imaging.
  • The probe's synergistic activation by ATP, biotin receptor targeting, and acidic environment contributes to its high efficacy.
  • This probe has potential for early and accurate cancer diagnosis.