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Related Concept Videos

Imprinting01:22

Imprinting

Behavioral imprinting is observed in some newborn animals and occurs when they develop strong and specific attachments to another animal (usually a parent) following brief, early-life exposures. Offspring imprint onto parents within a brief period after birth or hatching; this time window is called the critical period. Once imprinting occurs, the bond established between the parents and their offspring is usually long-lasting.
Enzyme-Linked Immunosorbent Assay01:33

Enzyme-Linked Immunosorbent Assay

In 1971, Peter Perlman and Eva Engvall developed an Enzyme-linked immunosorbent assay (ELISA or EIA). ELISA differs from western blot in that the assays are conducted in microtiter plates or in vivo rather than on an absorbent membrane.
There are many different types of ELISAs, but they all involve an antibody molecule whose constant region binds an enzyme, leaving the variable region free to bind its specific antigen.  Enzyme-substrate reaction allows the antigen to be visualized or quantified.
Genomic Imprinting and Inheritance02:30

Genomic Imprinting and Inheritance

Diploid organisms inherit genetic material through chromosomes from both parents. Copies of the same gene are known as alleles. In most cases, both alleles are simultaneously expressed and allow various cellular processes to function optimally. If one of the alleles is missing or mutated, the expression of the other allele can compensate; however, this is not true for all genes.
The expression of some genes depends on which parent passed the gene to the offspring, through a phenomenon known as...

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Updated: Jun 20, 2026

Ultrasensitive Detection of Biomarkers by Using a Molecular Imprinting Based Capacitive Biosensor
08:22

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Published on: February 16, 2018

Molecular imprinting-based pseudo-ELISA: recent advances.

Yuqin Ge1, Hongkang Wang2, Tianhai Ji1,2,3

  • 1Department of Pathology, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, 200011, P. R. China. skysea_ji@sina.com.

The Analyst
|June 19, 2026
PubMed
Summary
This summary is machine-generated.

Molecular imprinting creates artificial antibodies for pseudo-ELISA (pELISA), offering a stable, low-cost alternative. While not replacing traditional ELISA, pELISA shows promise for specific applications like point-of-care testing.

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Area of Science:

  • Analytical Chemistry
  • Materials Science
  • Biotechnology

Background:

  • Enzyme-linked immunosorbent assay (ELISA) relies on antibodies for analyte detection.
  • Antibodies have limitations: high cost, instability, and restricted use in extreme conditions or for small molecules.
  • Molecular imprinting offers a materials-based alternative for creating artificial recognition sites.

Purpose of the Study:

  • To review molecular imprinting-based pseudo-ELISA (pELISA) platforms.
  • To discuss current challenges, trends, and future prospects of pELISA.
  • To evaluate pELISA as a complement to traditional ELISA.

Main Methods:

  • Literature review of molecular imprinting-based pELISA over the last five years.
  • Analysis of the principles and applications of molecular imprinting in creating artificial antibodies.
  • Comparison of pELISA performance and characteristics with conventional ELISA.

Main Results:

  • Molecular imprinting enables the creation of polymers with artificial recognition sites (MIPs) mimicking antibody function.
  • MIPs offer potential advantages in stability, cost, and synthetic tunability compared to antibodies.
  • Several pELISA platforms utilizing MIPs have been reported, demonstrating feasibility.

Conclusions:

  • pELISA based on molecular imprinting is a promising technology.
  • It is unlikely to replace conventional ELISA soon but can complement it in niche areas.
  • Potential applications include low-cost point-of-care diagnostics and analysis in challenging environments.