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Tandem Mass Spectrometry01:21

Tandem Mass Spectrometry

Tandem mass spectrometry is a technique that uses multiple mass analyzers in series to obtain a higher selectivity and reduce chemical noise during analyte detection. Instruments with multiple analyzers separated by an interaction cell enable secondary fragmentation and selected study of the fragment ions.Secondary fragmentations occur in the interaction cell and can be induced by various factors. Fragmentation induced by collision with inert gases, such as N2, Ar, He, etc., is called...
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Mass spectrometry is an important technique for the identification of pure compounds. However, it has some limitations for the analysis of complex mixtures, often due to excessive fragmentation making the spectrum too complicated to decipher. Mass spectrometry can be combined with suitable separation methods in sequence, forming hyphenated methods, which are useful in the analysis of complex mixtures.
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Mass Spectrometry: Amine Fragmentation00:55

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Amines can be identified using mass spectroscopy based on their characteristic fragmentation patterns. The molecular ions of amines undergo fragmentation via ⍺-cleavage. The ⍺-cleavage of the carbon-carbon bonds in amines generates an alkyl radical and resonance-stabilized nitrogen-containing cation.
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Bioanalysis of Ametryn by UHPLC-MS3 Coupled With Multiple Fragmentation to Decrease Interference and Enhance

Yue Deng1,2, Hongjie Ju3, Fangyu Liu3

  • 1Central Hospital of Dalian University of Technology, Dalian University of Technology, Dalian, Liaoning, China.

Rapid Communications in Mass Spectrometry : RCM
|June 19, 2026
PubMed
Summary
This summary is machine-generated.

A new UHPLC-MS^3 method offers sensitive and selective quantification of the aquatic toxin ametryn in zebrafish tissues. This approach overcomes limitations of conventional methods, improving residue monitoring and toxicological studies.

Keywords:
UHPLC‐MS3ametrynmethod validationtissue distributionzebrafish

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Area of Science:

  • Environmental Chemistry
  • Analytical Chemistry
  • Toxicology

Background:

  • Ametryn poses risks to aquatic ecosystems.
  • Conventional methods for ametryn detection face challenges with matrix interference and sensitivity.
  • There is a need for improved analytical techniques for ametryn quantification in biological samples.

Purpose of the Study:

  • To develop and validate a highly selective and sensitive UHPLC-MS^3 method for ametryn quantification.
  • To apply the developed method for analyzing ametryn levels in various zebrafish tissues.
  • To establish a reliable platform for ametryn residue monitoring and toxicological research.

Main Methods:

  • A novel ultra-high-performance liquid chromatography-tandem mass spectrometry (UHPLC-MS^3) method utilizing electrospray ionization was established.
  • Quantification was performed using the MS^3 transition m/z 228.0 → 186.1 → 158.0.
  • Sample preparation and analysis were conducted on five zebrafish tissues (liver, muscle, brain, intestine, gill) with rapid chromatographic separation (2.8 min).

Main Results:

  • The UHPLC-MS^3 method significantly reduced matrix interference compared to MRM.
  • Signal intensity increased from 6.4 × 10^4 to 1.6 × 10^7 cps, and signal-to-noise ratio improved from 6.3 to 45.
  • The assay demonstrated excellent linearity (10-1000 pg/mL) and met FDA bioanalytical requirements for accuracy, precision, recovery, matrix effects, and stability.

Conclusions:

  • This study successfully implemented UHPLC-MS^3 for ametryn quantification in multiple zebrafish tissues.
  • The developed method provides a reliable and high-throughput platform for ametryn residue monitoring.
  • This technique supports mechanistic toxicology research by enabling accurate ametryn level determination in biological samples.