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Establishing the Epidemiological Cut-Off Value (ECOFF) for Cefquinome Against Staphylococcus aureus Using
Ijaz Ahmad1, Shakoor Ahmed1, Muhammad Faizan1
1College of Veterinary Sciences, The University of Agriculture, Peshawar, Pakistan.
Veterinary Medicine and Science
|June 19, 2026
Summary
This study establishes the epidemiological cut-off value for cefquinome against Staphylococcus aureus in cattle. These findings support antimicrobial resistance surveillance and guide cefquinome susceptibility testing in veterinary medicine.
Area of Science:
- Veterinary Microbiology
- Antimicrobial Resistance
- Bacterial Pathogenesis
Background:
- Staphylococcus aureus is a significant animal and human pathogen.
- Rising antimicrobial resistance necessitates robust surveillance tools.
- Cefquinome is a vital cephalosporin antibiotic in veterinary practice.
Purpose of the Study:
- To determine the wild-type (WT) cut-off value for cefquinome against S. aureus in cattle.
- To establish epidemiological cut-off values (ECVs) for cefquinome susceptibility testing.
- To support antimicrobial resistance surveillance in food-producing animals.
Main Methods:
- Isolated 110 S. aureus strains from cattle.
- Determined minimum inhibitory concentrations (MICs) using agar dilution and microdilution.
- Analyzed MIC distribution with goodness-of-fit testing and non-linear regression.
Main Results:
- Cefquinome MIC range was 0.03–2 µg/mL.
- Epidemiological cut-off value (ECV) determined as 2 µg/mL, covering 99.1% of the wild-type population.
- MIC of 0.5 µg/mL covered 95% of the distribution, indicating potential for resistance monitoring.
Conclusions:
- Established a scientific basis for cefquinome susceptibility interpretation in cattle.
- Provided critical data for antimicrobial resistance surveillance of S. aureus.
- Highlighted the need for further research into cefquinome pharmacokinetics in calves.
