Related Experiment Video
Updated: Jun 21, 2026
Surface-enhanced Resonance Raman Scattering Nanoprobe Ratiometry for Detecting Microscopic Ovarian Cancer via Folate Receptor Targeting
Published on: March 25, 2019
Intra-nanocomposite resonance energy transfer-based electrochemiluminescence biosensor for cervical cancer microRNA
Lin Peng1, Qian Zhang1, Liangwen Zhang1
1Department of Thyroid and Breast Surgery, and Institute of Rheumatology and Immunology, Affiliated Hospital of North Sichuan Medical College, Nanchong, 637000, China.
None:
In this work, an intra-nanocomposite resonance energy transfer-based electrochemiluminescence (RET-ECL) biosensor was developed for detecting microRNA (miRNA-21), a cervical cancer relative biomarker. By means of the in-situ reduction method, silver nanoparticles (Ag NPs) were uniformly coated on the surface of Zirconium-based metal organic frameworks (UiO-66-NH2) encapsulated with Ru(bpy)32+ (Ru@UiO-66-NH2), thus forming the Ru@UiO-66-NH2@Ag nanocomposite. Because of the incorporation of both ECL donor (Ru(bpy)32+) and acceptor (Ag NPs) within a single nanostructure, Ru@UiO-66-NH2@Ag showed high RET efficiency, causing a significantly quenched ECL response. Afterward, the presence of miRNA-21 activated the upstream toehold-mediated strand displacement reactions (TSDR) to generate abundant S strands, which could further trigger the downstream catalytic hairpin assembly (CHA). Immobilizing glucose oxidase (GOD) on the surface of Ru@UiO-66-NH2@Ag enabled the catalysis of glucose oxidation and then the produced H2O2 proceeded to etch Ag NPs to Ag+. As a result, a notably recovered ECL response was obtained for miRNA-21 assay with a low detection limit of 30 aM. Moreover, the biosensor successfully analyzed miRNA-21 expression levels in serum samples collected from cervical cancer patient and healthy volunteers, whose results were consistent with those of the standard methods. Therefore, this biosensor held huge potential in analysis of cervical cancer-related miRNA.
