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Reporter Genes02:11

Reporter Genes

Reporter genes are a type of protein-coding gene that are often tagged to a gene of interest. Once inside a target cell, reporter genes usually produce visually identifiable characteristics like fluorescence and luminescence when expressed along with the gene of interest. Thus, reporter genes “report” the presence or absence of genes of interest in an organism, determine the gene expression pattern, or track the physical location of a DNA segment or protein in the cell.
Commonly used reporter...

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  1. Home
  3. Enhancer Rna Transcription Near Segmentation Gene Enhancers Can Be Analyzed In Situ Using Fish.
  1. Home
  3. Enhancer Rna Transcription Near Segmentation Gene Enhancers Can Be Analyzed In Situ Using Fish.

Related Experiment Video

Mosaic Zebrafish Transgenesis for Evaluating Enhancer Sequences
07:23

Mosaic Zebrafish Transgenesis for Evaluating Enhancer Sequences

Published on: July 16, 2010

Enhancer RNA Transcription Near Segmentation Gene Enhancers Can Be Analyzed In Situ Using FISH.

Christine Mau1, Benjamin Schmid2, Ezzat El-Sherif3

  • 1Department of Evolutionary Developmental Genetics, Georg-August-University Göttingen, Justus-von-Liebig-Weg 11, 37077 Göttingen, Germany.

G3 (Bethesda, Md.)
|June 19, 2026

View abstract on PubMed

Summary
This summary is machine-generated.

Researchers developed a new imaging technique to study enhancer RNAs (eRNAs), non-coding transcripts involved in gene regulation. This method reveals how eRNA transcription dynamics influence gene expression in Drosophila embryos.

Keywords:
Drosophila melanogasterChromatin insulatorsEnhancer RNA (eRNA)FISH/HCR imagingMS2-MCP live imagingTranscriptional bursting

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Microinjection for Transgenesis and Genome Editing in Threespine Sticklebacks
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Microinjection for Transgenesis and Genome Editing in Threespine Sticklebacks

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Area of Science:

  • Molecular Biology
  • Developmental Biology
  • Genetics

Background:

  • Enhancer RNAs (eRNAs) are non-coding transcripts implicated in transcriptional regulation.
  • Previous studies relied on labor-intensive genomic methods, limiting mechanistic insights into eRNA function.
  • The precise roles of eRNA transcription and transcripts in gene regulation remain largely unknown.

Purpose of the Study:

  • To present a novel, imaging-based experimental approach for analyzing eRNA transcription characteristics.
  • To investigate the dynamics of eRNA expression and its relationship with mRNA production in Drosophila melanogaster embryos.
  • To explore the influence of regulatory elements like insulators on eRNA transcription.

Main Methods:

  • Combined high-sensitivity fluorescence in situ hybridization chain reaction (HCR) with high-resolution confocal microscopy.
  • Utilized anterior-posterior patterning genes as a model system in fixed and live Drosophila embryos.
  • Employed MS2-MCP live imaging to analyze mRNA dynamics in response to insulator activity.

    • Main Results:

    • Identified transcriptional foci generating eRNAs correlated with mRNA production at the same locus.
    • Demonstrated that eRNA transcription is independent of promoter activity and can be influenced by insulators.
    • Observed eRNAs originating both within and outside canonical enhancer regions, influenced by local regulatory context.

    Conclusions:

    • The novel imaging approach provides a powerful platform for dissecting enhancer-eRNA interactions.
    • eRNA transcription initiation is sensitive to local regulatory context and can occur outside classical enhancers.
    • Insulators modulate eRNA transcription and impact mRNA expression dynamics, suggesting a role in gene regulation.