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Related Concept Videos

Cell Specific Gene Expression01:58

Cell Specific Gene Expression

Multicellular organisms contain a variety of structurally and functionally distinct cell types, but the DNA in all the cells originated from the same parent cells. The differences in the cells can be attributed to the differential gene expression. Liver cells, whose functions include detoxification of blood, production of bile to metabolize fats, and synthesis of proteins essential for metabolism, must express a specific set of genes to perform their functions. Gene expression also varies with...

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Methods for the Modulation and Analysis of NF-κB-dependent Adult Neurogenesis
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Dissecting hypertonicity- and NFAT5-dependent gene expression programs in mpkCCD cells.

Kristina Engel1, Dmitry Chernyakov1, Moritz Pernecker2

  • 1Department of Medicine, Hematology and Oncology, Martin Luther University Halle-Wittenberg, Halle (Saale), Germany.

Physiological Reports
|June 20, 2026
PubMed
Summary
This summary is machine-generated.

Nuclear factor of activated T-cells 5 (NFAT5) regulates gene expression in kidney cells. This study reveals NFAT5

Keywords:
NFAT5hypertonicitympkCCD

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Area of Science:

  • Renal physiology and molecular biology
  • Kidney gene expression regulation

Background:

  • The corticomedullary osmotic gradient influences kidney gene expression.
  • Nuclear factor of activated T-cells 5 (NFAT5) is activated by hypertonicity and regulates osmoprotective genes.
  • NFAT5's role in basal gene regulation is not fully understood.

Purpose of the Study:

  • To investigate the role of NFAT5 in gene regulation under both isotonic and hypertonic conditions.
  • To identify genes regulated by NFAT5 in kidney cells.
  • To compare in vitro findings with in vivo mouse models.

Main Methods:

  • Utilized murine principal kidney cortical collecting duct (mpkCCD) cells.
  • Induced functional deletion of NFAT5 in mpkCCD cells.
  • Performed gene expression profiling under isotonic and hypertonic conditions.
  • Compared transcriptomes with gene expression data from NFAT5 knockout mice.

Main Results:

  • Hypertonic stress caused significant transcriptional changes in control cells, altered in NFAT5-deficient cells.
  • A partial overlap was observed between hypertonicity-associated, NFAT5-dependent gene expression in vitro and in vivo.
  • Known NFAT5 target genes, such as Aqp2 and Ranbp3l, were downregulated in NFAT5-deficient conditions.

Conclusions:

  • NFAT5 plays a role in regulating gene expression under both basal and hypertonic conditions.
  • mpkCCD cells serve as a valuable complementary model for studying NFAT5-mediated gene regulation in vitro.
  • Findings provide insights into the spatial gene expression patterns in the renal medulla.