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Updated: Jun 21, 2026

Dual-Color Fluorescence Cross-Correlation Spectroscopy to Study Protein-Protein Interaction and Protein Dynamics in Live Cells
Published on: December 11, 2021
Current Status and Trends of Two-Dimensional Correlation Spectroscopy (2D-COS)
Yeonju Park1, Sujin Lee1, Isao Noda2
1Department of Chemistry, Institute for Molecular Science and Fusion Technology, and Kangwon Radiation Convergence Research Support Center, Kangwon National University, Chuncheon 24341, Korea.
None:
This comprehensive survey reviews the trajectory of research dissemination in the field of two-dimensional correlation spectroscopy (2D-COS) from 1 July 2023 to 10 February 2025. It provides a systematic overview of books, reviews, tutorials, and patent applications, alongside a vast array of innovative theoretical concepts and diverse applications that had emerged during this period. Beyond reporting advancements, this review critically examines instances of improper usage or citations of 2D-COS to promote methodological rigor within the community. The survey highlights significant innovations in probe integration, chemometric data analysis, and technical methodologies. These developments underscore the expanding role of 2D-COS in materials science, environmental monitoring, and complex biological systems. By synthesizing recent literature, this review illustrates dynamic growth, increasing refinement, and multi-disciplinary diversification of 2D-COS across the global scientific landscape.
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