Current Status and Trends of Two-Dimensional Correlation Spectroscopy (2D-COS)
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Updated: Jun 21, 2026

Dual-Color Fluorescence Cross-Correlation Spectroscopy to Study Protein-Protein Interaction and Protein Dynamics in Live Cells
Published on: December 11, 2021
Yeonju Park1, Sujin Lee1, Isao Noda2
1Department of Chemistry, Institute for Molecular Science and Fusion Technology, and Kangwon Radiation Convergence Research Support Center, Kangwon National University, Chuncheon 24341, Korea.
Abstract:
This comprehensive survey reviews the trajectory of research dissemination in the field of two-dimensional correlation spectroscopy (2D-COS) from 1 July 2023 to 10 February 2025. It provides a systematic overview of books, reviews, tutorials, and patent applications, alongside a vast array of innovative theoretical concepts and diverse applications that had emerged during this period. Beyond reporting advancements, this review critically examines instances of improper usage or citations of 2D-COS to promote methodological rigor within the community. The survey highlights significant innovations in probe integration, chemometric data analysis, and technical methodologies. These developments underscore the expanding role of 2D-COS in materials science, environmental monitoring, and complex biological systems. By synthesizing recent literature, this review illustrates dynamic growth, increasing refinement, and multi-disciplinary diversification of 2D-COS across the global scientific landscape.
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