Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: Jun 23, 2026

Detection and Monitoring of Tumor Associated Circulating DNA in Patient Biofluids
06:53

Detection and Monitoring of Tumor Associated Circulating DNA in Patient Biofluids

Published on: June 8, 2019

PVA sponge method improves detection efficiency of cfDNA in plasma.

Madoka Kisoi1,2, Kotaro Yamashita3, Kenji Kinoshita2

  • 1Department of Gastroenterological Surgery, The University of Osaka Graduate School of Medicine, 2-2, Yamada-Oka, Suita City, Osaka, 565-0871, Japan.

International Journal of Clinical Oncology
|June 20, 2026
PubMed
Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

SIRT3-Mediated SOD2 and PGC-1α Contribute to Chemoresistance in Colorectal Cancer Cells.

Annals of surgical oncology·2021
Same author

A case of single-incision laparoscopic surgery for acute appendicitis with left ventricular assist device.

Asian journal of endoscopic surgery·2021
Same author

Limited resection vs. pancreaticoduodenectomy for primary duodenal adenocarcinoma: a systematic review and meta-analysis.

International journal of clinical oncology·2021
Same author

Taste alteration after gastrectomy in patients with gastric cancer.

Surgery today·2021
Same author

Hepatic Stellate Cells in Hepatocellular Carcinoma Promote Tumor Growth Via Growth Differentiation Factor 15 Production.

Gastroenterology·2020
Same author

Resolution of Pancreatic Duct Dilatation After Resection of Main-Duct Intraductal Papillary Mucinous Neoplasm.

The American surgeon·2020

A novel polyvinyl alcohol (PVA) sponge method allows direct quantification of cell-free DNA (cfDNA) from plasma using quantitative PCR (qPCR) without prior extraction, offering a faster and simpler approach for liquid biopsies.

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Genomics

Background:

  • Cell-free DNA (cfDNA) analysis is crucial for molecular diagnostics and research.
  • Conventional cfDNA extraction methods are labor-intensive and inefficient due to blood-derived substance interference.
  • A novel method using polyvinyl alcohol (PVA) sponges for direct cfDNA quantification was developed.

Purpose of the Study:

  • To develop a simplified and efficient method for cfDNA analysis.
  • To enable direct quantitative PCR (qPCR) of cfDNA from plasma without extraction or purification.
  • To evaluate the performance of the PVA sponge method compared to commercial kits.

Main Methods:

  • PVA sponge pieces were used to dry plasma samples.
  • The dried PVA sponges were directly added to qPCR mixtures.
Keywords:
Esophageal cancerLiquid biopsyPVA spongecfDNA

More Related Videos

Detection of Cell-Free DNA in Blood Plasma Samples of Cancer Patients
08:25

Detection of Cell-Free DNA in Blood Plasma Samples of Cancer Patients

Published on: September 9, 2020

Techniques for the Analysis of Extracellular Vesicles Using Flow Cytometry
09:39

Techniques for the Analysis of Extracellular Vesicles Using Flow Cytometry

Published on: March 17, 2015

Related Experiment Videos

Last Updated: Jun 23, 2026

Detection and Monitoring of Tumor Associated Circulating DNA in Patient Biofluids
06:53

Detection and Monitoring of Tumor Associated Circulating DNA in Patient Biofluids

Published on: June 8, 2019

Detection of Cell-Free DNA in Blood Plasma Samples of Cancer Patients
08:25

Detection of Cell-Free DNA in Blood Plasma Samples of Cancer Patients

Published on: September 9, 2020

Techniques for the Analysis of Extracellular Vesicles Using Flow Cytometry
09:39

Techniques for the Analysis of Extracellular Vesicles Using Flow Cytometry

Published on: March 17, 2015

  • Recovery yields and qPCR performance were assessed using DNA standards and clinical plasma samples, compared against two commercial kits (DBM and MEC).

    • Main Results:

    • PVA sponges did not inhibit qPCR, yielding 90.7%-105.3% recovery for genomic DNA.
    • The PVA sponge method mitigated qPCR inhibition in plasma, improving amplification and reducing Ct variability.
    • For low-concentration DNA (10^1 copies/μL), PVA sponge recovery (46.0%) significantly outperformed commercial kits (11.4%-30.9%).
    • The PVA sponge method required only four steps and approximately one hour.
    • Median cfDNA concentration in patient plasma was 7.8 copies/μL.

    Conclusions:

    • The PVA sponge method allows for efficient and rapid cfDNA quantification directly from plasma without extraction.
    • This method simplifies the process, reduces hands-on time, and is cost-effective.
    • The PVA sponge technique is a promising tool for liquid biopsy applications in clinical settings.