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Avian Influenza Surveillance with FTA Cards: Field Methods, Biosafety, and Transportation Issues Solved
Published on: August 2, 2011
Isolation, identification and pathogenicity analysis of a virulent duck enteritis virus strain causing outbreak in
Shuhui Sang1, Haoqian Wang1, Yuqing Dan1
1College of Veterinary Medicine, Henan Agricultural University, Zhengzhou 450046, China.
Abstract:
Duck viral enteritis, caused by duck plague virus, is a highly lethal infectious disease that continuously causes substantial economic losses to the global duck industry. In this study, a duck plague virus strain designated HNSQ0920 was isolated and purified from a vaccinated duck farm in Henan Province, China. Whole-genome sequencing and phylogenetic analysis revealed that HNSQ0920 clusters within the predominant virulent duck plague virus lineage currently circulating in China. Amino acid variation analysis identified mutations in four key virulence-associated proteins, including UL22 (F119L), UL41 (G44E), UL47 (A36V), and US7 (I168N). Further pathogenicity assays demonstrated that this isolate could infect and kill ducklings younger than 30 days of age, with a 100% mortality rate in contact-infected ducks. Collectively, these results suggested that HNSQ0920 is a potentially highly transmissible and virulent field variant strain. The present findings provide critical insights for duck plague virus vaccine development and disease prevention and control, and highlight an urgent need to strengthen biosecurity measures in duck production systems on farms within endemic regions.
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