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Updated: Jun 23, 2026
Multiplexed Isothermal Amplification Based Diagnostic Platform to Detect Zika, Chikungunya, and Dengue 1
Published on: March 13, 2018
Pan- India molecular surveillance of dengue serotypes, 2023-2025
Gururaj Rao N Deshpande1, Nitali Tadkalkar1, Jitendra Narayan2
1ICMR-National Institute of Virology, Pune, Maharashtra, India.
Background:
The four serotypes of dengue virus are a significant public health challenge in India, with regional variability in serotype circulation and limited comprehensive spacio-temporally harmonized data across diverse geographic areas. Thus, a nation-wide pan-India passive molecular surveillance study was conducted in 45 of 165 Virus Research and Diagnostic Laboratories (VRDLs), in 25 states and union territories between 2023 and 2025.
Methods:
A total of 6889 dengue positive serum samples referred to the VRDLs (n = 45) for diagnosis of dengue were tested by Realtime reverse transcriptase PCR for determination of infecting serotypes.
Results:
All regions showed co-circulation of multiple serotypes, with eight states having all four serotypes co-circulating. DENV-2 was predominant during the study period. Increased likelihood of thrombocytopenia, arthralgia, and hemorrhagic manifestations were directly associated with concurrent infections with multiple serotypes compared to mono-serotypic infections.
Conclusion:
These findings underscore the importance of continuous, geographically representative molecular surveillance to monitor evolving dengue serotype dynamics, informing outbreak prediction and guiding dengue vaccine introduction strategies in India.
