Related Experiment Video
Updated: Jun 23, 2026
10:25
Real-time Bioluminescence Imaging of Notch Signaling Dynamics during Murine Neurogenesis
Published on: December 12, 2019
Sbno1 and Usp8 Cooperate to Enhance Notch Signaling in Regulating Neural Stem Cells
Oyunchimeg Naranbaatar1, Dai Ihara1, Hidenori Tabata2
1Division of Neuroanatomy, Department of Anatomy, Shiga University of Medical Science, Otsu, Japan.
Development, Growth & Differentiation
|June 20, 2026
Summary
Strawberry Notch homolog 1 (Sbno1) and Usp8 are crucial for maintaining neural stem cells (NSCs) and proper brain development. They regulate the Notch signaling pathway, essential for NSC self-renewal and differentiation.
Area of Science:
- Neuroscience
- Developmental Biology
- Molecular Biology
Background:
- The Notch signaling pathway is vital for neural stem cell (NSC) functions, including self-renewal and differentiation.
- The role of Strawberry Notch homolog 1 (Sbno1), a Drosophila gene homolog, in mammalian NSCs is not well understood.
Purpose of the Study:
- To investigate the function of Sbno1 and its interaction with Usp8 in mammalian neural stem cells.
- To elucidate the molecular mechanisms by which Sbno1 and Usp8 regulate Notch signaling in NSCs.
Main Methods:
- Interaction studies between Sbno1, Rbpj, and Usp8.
- Promoter analyses to assess gene expression.
- In utero electroporation to manipulate Usp8 and Sbno1 levels in embryonic cortical NSCs.
Main Results:
- Sbno1 interacts with Rbpj and Usp8, synergistically enhancing Hes5 expression in NSCs.
- Usp8 is essential for NSC maintenance; its absence impairs NSC maintenance.
- Loss of Sbno1 leads to NSC depletion and premature neuronal differentiation.
Conclusions:
- Sbno1 and Usp8 are critical regulators of Notch signaling in mammalian NSCs.
- These proteins play essential physiological roles in mammalian cortical development.
- Sbno1 stabilizes the Notch intracellular domain and resolves R-loops at the Hes5 promoter.
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The Notch gene came into the limelight in 1914 after the discovery that its mutation in Drosophila melanogaster leads to a serrated (or "notched") wing margin phenotype. It was not until 1985...
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