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Related Experiment Video

Updated: Jun 23, 2026

A Bright NIR-II Fluorescence Probe for Vascular and Tumor Imaging
05:51

A Bright NIR-II Fluorescence Probe for Vascular and Tumor Imaging

Published on: March 17, 2023

White-Light-Excitable Deep-Red/NIR Organic Afterglow Nanoparticles for High-Contrast In Vivo Imaging.

Zongliang Xie1, Bowen Li2,3, Chongzhi Wu3,4

  • 1Institute for Functional Intelligent Materials, National University of Singapore, Singapore, Singapore.

Angewandte Chemie (International Ed. in English)
|June 21, 2026
PubMed
Summary

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Researchers developed novel organic afterglow probes for deep-tissue bioimaging. These probes offer long-lived near-infrared phosphorescence activated by visible light, enabling high-contrast in vivo imaging.

Area of Science:

  • Materials Science
  • Chemistry
  • Biomedical Engineering

Background:

  • Near-infrared (NIR) organic afterglow probes are crucial for deep-tissue bioimaging.
  • Challenges exist in achieving white-light excitation, long NIR phosphorescence, and uniform nanoparticle fabrication simultaneously.

Purpose of the Study:

  • To develop a bottom-up approach for creating lattice-matched host-guest nanocrystals for bioimaging.
  • To achieve deep-red/NIR afterglow with long lifetimes and efficient phosphorescence quantum yields.

Main Methods:

  • Incorporation of a rigid phenylcarbazole host (BMC) with extended-conjugation guests (PyC or BPC) into lattice-matched host-guest nanocrystals.
  • Utilizing a bottom-up nanoprecipitation method for fabricating monodisperse phosphorescent nanoparticles.
Keywords:
deep‐tissue bioimagingnear‐infrared emissionorganic afterglowroom‐temperature phosphorescencewhite‐light‐excitable

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Last Updated: Jun 23, 2026

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  • Activation by visible light up to 475 nm.

    • Main Results:

    • Achieved guest-dominated deep-red/NIR afterglow with emission up to 762 nm.
    • Observed an ultralong phosphorescence lifetime of 126.1 ms and a quantum yield of up to 2.7%.
    • Fabricated monodisperse phosphorescent nanoparticles with negligible cytotoxicity via bottom-up nanoprecipitation.

    Conclusions:

    • The developed lattice-matched nanocrystals enable bright, persistent deep-red/NIR emission under white-light excitation.
    • The bottom-up fabrication method overcomes challenges in nanoparticle size heterogeneity and material loss.
    • These nanoparticles are promising for high-contrast in vivo bioimaging applications.