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Related Experiment Video

Updated: Jun 23, 2026

Generating Self-Assembling Human Heart Organoids Derived from Pluripotent Stem Cells
08:56

Generating Self-Assembling Human Heart Organoids Derived from Pluripotent Stem Cells

Published on: September 15, 2021

Cardiovascular Organoids With Adjustable Endothelial Composition via SOX17-Engineered hPSCs.

Po-Yu Liang1,2, Gyuhyung Jin1,2, Nathan R Petrucci3

  • 1Davidson School of Chemical Engineering, Purdue University, West Lafayette, Indiana, USA.

Biotechnology and Bioengineering
|June 21, 2026
PubMed
Summary

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This summary is machine-generated.

Researchers developed advanced cardiovascular organoids from genome-edited stem cells. These 3D models accurately replicate human heart conditions and drug responses, offering a new tool for heart disease research.

Area of Science:

  • Cardiovascular Research
  • Stem Cell Biology
  • Organoid Technology

Background:

  • Organoids represent a novel platform for human biology and health research.
  • Organoids enable the study of complex 3D structures and multicellular interactions.
  • Cardiovascular organoids offer a reliable model for studying heart conditions.

Purpose of the Study:

  • To generate an advanced cardiovascular organoid using a genome-edited human pluripotent stem cell line.
  • To achieve controlled endothelial specification, adjustable cell-type composition, and human heart-like morphology.
  • To establish a robust and easy-to-produce platform for studying human heart disease.

Main Methods:

  • Utilized a genome-edited human pluripotent stem cell line with inducible SOX17 expression.
Keywords:
SOX17cardiomyocytecardiovascular diseasegenome editinghuman pluripotent stem cellorganoid

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Vascular Organoid Generation from Human-Induced Pluripotent Stem Cells
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Vascular Organoid Generation from Human-Induced Pluripotent Stem Cells

Published on: December 13, 2024

Related Experiment Videos

Last Updated: Jun 23, 2026

Generating Self-Assembling Human Heart Organoids Derived from Pluripotent Stem Cells
08:56

Generating Self-Assembling Human Heart Organoids Derived from Pluripotent Stem Cells

Published on: September 15, 2021

Vascular Organoid Generation from Human-Induced Pluripotent Stem Cells
04:41

Vascular Organoid Generation from Human-Induced Pluripotent Stem Cells

Published on: December 13, 2024

  • Generated 3D cardiovascular organoids with controllable cellular composition and heart-like structures.
  • Tested organoid response to doxorubicin, cryoinjury-induced myocardial infarction, and isoproterenol.

    • Main Results:

    • Organoids recapitulated cardiotoxic effects of doxorubicin, showing decreased viability and contractile activity.
    • Myocardial infarction model showed reduced beating, viability, and α-actinin expression, with increased fibroblast formation, mitigated by Captopril.
    • Isoproterenol treatment demonstrated effects on Ca2+ transient amplitude and action potential duration, consistent with β-adrenergic responses.

    Conclusions:

    • Established a protocol for generating in vitro 3D cardiovascular organoids.
    • Developed a controllable and reproducible platform for cardiovascular research.
    • The cardiovascular organoid model provides a robust tool for future studies of human heart disease.