Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Three-Dimensional Microscopy in Microbiology01:28

Three-Dimensional Microscopy in Microbiology

Three-dimensional imaging techniques are essential in cell biology, allowing researchers to visualize intricate cellular structures with high resolution. Two prominent methods, Differential Interference Contrast Microscopy (DIC) and Confocal Scanning Laser Microscopy (CSLM), provide distinct advantages for imaging live and thick specimens, respectively.Differential Interference Contrast MicroscopyDIC microscopy enhances contrast in transparent, unstained samples by converting phase...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The multifaceted regulatory network of microRNA396: Orchestrating plant plasticity through coordinating development and various stresses.

Journal of integrative plant biology·2026
Same author

Reply to: prophylactic esketamine to reduce postpartum depression in primiparae: A multicentre, double-blind, randomised clinical trial.

European journal of anaesthesiology·2026
Same author

Numerical analysis of three-dimensional middle ear and inner ear based on nonlinear and viscoelastic constitutive relations.

Mechanobiology in medicine·2026
Same author

Hybrid Neural Network-Based PDR with Multi-Layer Heading Correction Across Smartphone Carrying Modes.

Sensors (Basel, Switzerland)·2026
Same author

Efficacy of tegileridine combined with intercostal nerve block for postoperative analgesia following thoracoscopic pulmonary resection: a study protocol for a randomised controlled trial.

BMJ open·2026
Same author

Comment on "Associations of accelerometer-measured sedentary behavior and physical activity with sleep in older adults".

Journal of the Formosan Medical Association = Taiwan yi zhi·2026

Related Experiment Video

Updated: Jun 23, 2026

Imaging the Root Hair Morphology of Arabidopsis Seedlings in a Two-layer Microfluidic Platform
09:23

Imaging the Root Hair Morphology of Arabidopsis Seedlings in a Two-layer Microfluidic Platform

Published on: August 15, 2017

A three-dimensional reconstruction method for seedlings based on improved DIFIX3D.

Zibo Yin1, Tingting Yang1, Suyin Zhou1

  • 1College of Mathematics and Computer Science, Zhejiang Agriculture and Forestry University, Hangzhou, 311800, China.

Plant Methods
|June 21, 2026
PubMed
Summary
This summary is machine-generated.

This study introduces an improved 3D seedling reconstruction method using Stable Diffusion-VAE within the DIFIX3D+ framework. The new approach enhances geometric accuracy and visual fidelity, overcoming limitations of existing techniques for complex plant structures.

Keywords:
3D reconstruction3DGS (3D Gaussian splatting)Image segmentationSeedlings

More Related Videos

A Simple Chamber for Long-term Confocal Imaging of Root and Hypocotyl Development
07:59

A Simple Chamber for Long-term Confocal Imaging of Root and Hypocotyl Development

Published on: May 17, 2017

Using High Resolution Computed Tomography to Visualize the Three Dimensional Structure and Function of Plant Vasculature
11:49

Using High Resolution Computed Tomography to Visualize the Three Dimensional Structure and Function of Plant Vasculature

Published on: April 5, 2013

Related Experiment Videos

Last Updated: Jun 23, 2026

Imaging the Root Hair Morphology of Arabidopsis Seedlings in a Two-layer Microfluidic Platform
09:23

Imaging the Root Hair Morphology of Arabidopsis Seedlings in a Two-layer Microfluidic Platform

Published on: August 15, 2017

A Simple Chamber for Long-term Confocal Imaging of Root and Hypocotyl Development
07:59

A Simple Chamber for Long-term Confocal Imaging of Root and Hypocotyl Development

Published on: May 17, 2017

Using High Resolution Computed Tomography to Visualize the Three Dimensional Structure and Function of Plant Vasculature
11:49

Using High Resolution Computed Tomography to Visualize the Three Dimensional Structure and Function of Plant Vasculature

Published on: April 5, 2013

Area of Science:

  • Computer Vision
  • Computational Biology
  • 3D Reconstruction

Background:

  • Three-dimensional (3D) reconstruction of seedlings faces challenges with occlusion and insufficient data, leading to geometric inaccuracies and rendering artifacts.
  • Existing methods often rely on pixel-level constraints, proving unstable for slender, complex plant structures.
  • Limitations include poor stability and susceptibility to noise from lighting, reflections, and texture repetition.

Purpose of the Study:

  • To develop an advanced 3D reconstruction method for seedlings that improves accuracy and stability, particularly for complex structures.
  • To enhance the constraint mechanism in 3D reconstruction by incorporating representation-level supervision.
  • To provide a high-quality, low-cost technical solution for 3D seedling reconstruction in intricate environments.

Main Methods:

  • An improved DIFIX3D+ framework integrating the Stable Diffusion-Variational Autoencoder (VAE) module.
  • High-precision seedling segmentation using Florence-2 semantic priors and Segment Anything Model 2 (SAM2).
  • Latent space consistency supervision by jointly mapping restored and rendered images, complementing pixel-level constraints.

Main Results:

  • Significant improvements in Peak Signal-to-Noise Ratio (PSNR) by 28.99% over 3D Gaussian Splatting (3DGS) and 24.25% over DIFIX3D+.
  • Enhanced Structural Similarity Index Measure (SSIM) by 3.16% over 3DGS and 3.74% over DIFIX3D+.
  • Reduced Learned Perceptual Image Patch Similarity (LPIPS) by 9.24% and 41.94%, respectively, demonstrating superior reconstruction quality.

Conclusions:

  • The proposed method achieves superior 3D reconstruction accuracy and geometric fidelity for seedlings compared to state-of-the-art models.
  • Integration of representation-level constraints effectively suppresses noise and improves stability in complex scenes.
  • The method offers a promising technical reference for high-quality, cost-effective 3D seedling reconstruction.