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Updated: Jun 23, 2026

Epithelial Cell Infection Analyses with Shigella
04:56

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Published on: February 9, 2024

Deciphering Distinct Pathogenetic Strategies Employed by Shigella Serotypes Using Human Intestinal Organoids.

Nidhi Kamboj1, Balvinder Mohan1, Sadhna B Lal2

  • 1Department of Medical Microbiology, Post Graduate Institute of Medical Education and Research, Chandigarh, 160012 India.

Indian Journal of Microbiology
|June 22, 2026
PubMed
Summary
This summary is machine-generated.

Human intestinal organoids effectively model Shigella invasion, revealing S. dysenteriae as the most invasive species. This organoid system aids in understanding Shigella pathogenesis and developing new vaccines.

Keywords:
EnteroidsInfection cycleInvasivenessPathogenesisShigella

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Published on: September 10, 2021

Area of Science:

  • Microbiology
  • Pathogenesis
  • Infectious Diseases

Background:

  • Shigella species cause significant diarrheal illness globally.
  • Understanding Shigella invasion mechanisms is crucial for developing effective treatments and vaccines.
  • Existing models may not fully recapitulate human intestinal environments for studying Shigella pathogenesis.

Purpose of the Study:

  • To develop and validate a human intestinal organoid model for assessing Shigella invasion.
  • To compare the invasive abilities of different Shigella species (S. dysenteriae, S. flexneri, S. sonnei).
  • To investigate Shigella-induced epithelial damage and virulence gene expression (ipaH) in the organoid model.

Main Methods:

  • Human intestinal organoids (enteroids) were established from human intestinal biopsies.
  • Enteroids were infected with S. dysenteriae, S. flexneri, and S. sonnei.
  • Gentamicin protection assays quantified bacterial invasion.
  • Bright field and immunofluorescence microscopy visualized Shigella entry and host cell morphology.
  • Differential expression of the ipaH gene was analyzed.

Main Results:

  • Human intestinal organoids provide a feasible model for studying Shigella pathogenesis.
  • S. dysenteriae demonstrated significantly higher invasive ability compared to S. flexneri and S. sonnei.
  • Microscopy revealed distinct invasion patterns and morphological changes induced by Shigella species.
  • ipaH gene expression correlated with the observed invasive capacities of different Shigella species.

Conclusions:

  • The human intestinal organoid model accurately reflects differential Shigella invasion capabilities.
  • This model system is suitable for identifying novel Shigella antigens and for vaccine development.
  • Findings contribute to a better understanding of Shigella pathogenesis and diarrheal disease control, particularly in high-burden regions.