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Related Experiment Video

Updated: Jun 23, 2026

Vibrodissociation of Neurons from Rodent Brain Slices to Study Synaptic Transmission and Image Presynaptic Terminals

Vibrodissociation of Neurons from Rodent Brain Slices to Study Synaptic Transmission and Image Presynaptic Terminals

Published on: May 25, 2011

SynCAR platform for capturing neuronal synaptic proteopathic seeds.

Rebecca M Sebastian¹, Robert Kupp², Dhaval Nanavati³

¹AbbVie, Cambridge Research Center, 200 Sidney Street, Cambridge, MA 02139, USA.

|June 22, 2026

Summary

This summary is machine-generated.

Researchers developed a novel synaptic chimeric antigen receptor (synCAR) to capture and study synaptic proteins. This tool aids in understanding neurodegenerative diseases like Alzheimer's by targeting pathological tau species.

Keywords:

Cell biology Molecular biology Neuroscience

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Last Updated: Jun 23, 2026

Vibrodissociation of Neurons from Rodent Brain Slices to Study Synaptic Transmission and Image Presynaptic Terminals

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Published on: May 25, 2011

Preparation of Synaptoneurosomes from Mouse Cortex using a Discontinuous Percoll-Sucrose Density Gradient

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Published on: September 17, 2011

Microtransplantation of Synaptic Membranes to Reactivate Human Synaptic Receptors for Functional Studies

Microtransplantation of Synaptic Membranes to Reactivate Human Synaptic Receptors for Functional Studies

Published on: July 20, 2022

Area of Science:

Neuroscience
Molecular Biology
Biochemistry

Background:

Synaptic proteins are crucial for neuronal function but implicated in the spread of neurodegenerative diseases.
Existing technologies face limitations in identifying synaptic proteins in situ.
Pathological proteins, like tau in Alzheimer's disease, can spread between synapses.

Purpose of the Study:

To develop a novel tool for the in situ identification and modulation of synaptic proteins.
To investigate the role of synaptic proteins in the prion-like spreading of pathological tau.
To establish a platform for neurodegenerative disease research and drug development.

Main Methods:

Construction of a modular synaptic chimeric antigen receptor (synCAR) by fusing a single-chain antibody fragment with neurolignin-1 (NLGN1).
Application of the synCAR platform to capture pathogenic tau species at the synapse using the PHF1 antibody.
Expression of the PHF1 synCAR in mouse primary and human neuronal tau seeding models.

Main Results:

The synCAR platform successfully captured pathogenic synaptic tau species.
Anchoring the tau antibody PHF1 to the synapse via synCAR enhanced tau aggregation.
Concentration of pathological tau seeds at the synapse by synCAR likely increased aggregation.

Conclusions:

The study presents the first method for isolating and modulating synaptic protein function in situ.
The synCAR platform is a valuable tool for synaptic protein research.
SynCAR technology holds promise for neurodegenerative disease drug development, particularly for Alzheimer's disease.