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Gokce Alp¹, Behnoush Kermanshahi¹, Andrea Olaizola²

¹Applied Research Centre, Langara College, Vancouver V5Y 2Z6, British Columbia, Canada.

|June 22, 2026

Summary

This summary is machine-generated.

Accurate quantification of phytocannabinoids is crucial for drug development. This study compares HPLC, UV/vis Spectrophotometry, and Fast Blue B Salt colorimetric analysis to determine the best method for cannabinoid quantitation.

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Area of Science:

Pharmacology
Analytical Chemistry
Drug Development

Background:

Isolated phytocannabinoids show therapeutic potential, driving interest in their drug development.
Accurate quantification of phytocannabinoids is essential for in vitro drug efficacy and pharmacokinetic studies.
Low solubility and plastic adsorption complicate cannabinoid analysis in biological and formulation matrices.

Purpose of the Study:

To compare three analytical methods for accurate and efficient phytocannabinoid quantitation.
To evaluate the utility of HPLC, UV/vis Spectrophotometry, and Fast Blue B Salt (FBBS) colorimetric analysis for cannabinoid analysis.
To identify challenges and advantages of each method for advancing cannabinoid drug development.

Main Methods:

High Performance Liquid Chromatography (HPLC) was employed for cannabinoid separation and quantification.
UV/vis Spectrophotometry was used to assess cannabinoid concentrations based on light absorbance.
Colorimetric analysis utilizing Fast Blue B Salt (FBBS) was performed for cannabinoid detection.

Main Results:

Each method (HPLC, UV/vis, FBBS) presented distinct advantages and limitations for cannabinoid quantitation.
The choice of analytical method impacts the accuracy and efficiency of dose-response and pharmacokinetic profiling.
Understanding method-specific challenges is key for reliable phytocannabinoid research.

Conclusions:

Selecting an appropriate analytical method is a critical step in phytocannabinoid drug development.
Comprehensive understanding of HPLC, UV/vis, and FBBS methods aids in advancing therapeutic cannabinoid research.
Accurate and efficient cannabinoid quantitation is vital for unlocking the pharmacological potential of phytocannabinoids.