Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Recrystallization: Solid–Solution Equilibria01:10

Recrystallization: Solid–Solution Equilibria

Recrystallization is a purification technique used to separate impurities from solid compounds. In this technique, no chemical reactions occur. Instead, it exploits physical properties only, specifically, the solubility differences between the desired compound and impurities, either at a single temperature or at different temperatures, and under other selected conditions. The solid-solution equilibrium (solubility equilibrium) of each component in the solution represents a binary phase...
Downstream Processing01:29

Downstream Processing

Downstream processing begins once fermentation is complete and involves a series of steps to recover and purify products such as acids, vitamins, antibiotics, or proteins.Cell HarvestingFor example, for intracellular protein-based products, the first step is harvesting the cells. This is typically achieved using centrifugation or filtration to separate the cells from the liquid phase.Cell Disruption for Intracellular ProductsIf the target product is intracellular, the harvested cells must be...
Upstream Processing01:27

Upstream Processing

Upstream processing represents a critical phase in biomanufacturing, wherein biological systems such as microorganisms, mammalian cells, or insect cells are cultivated to produce therapeutic proteins, vaccines, enzymes, or other biologically derived products. This phase encompasses all steps from the selection and genetic manipulation of the production organism to the cultivation of cells in bioreactors under tightly controlled environmental conditions.Host Selection and Genetic OptimizationThe...
Precipitation Processes01:12

Precipitation Processes

The experimental conditions in a gravimetric analysis should be optimized to maximize the particle size and purity of the obtained precipitate. Ideally, the concentration of the precipitating reagent should be low with effective stirring to maintain low relative supersaturation for the growth of large crystals. In homogeneous precipitation, the precipitant is slowly generated by a chemical reaction in the solution to avoid local reagent excesses. For example, urea decomposes gradually to...
Production of Alcohol01:27

Production of Alcohol

Continuous fermentation is a key strategy in industrial ethanol production, particularly when efficiency, scalability, and high yields are essential. This approach allows for uninterrupted operation and optimized resource utilization. The primary feedstock, corn starch, undergoes enzymatic hydrolysis facilitated by α-amylase and glucoamylase. These enzymes break down the starch into fermentable sugars such as glucose, which are readily assimilated by fermentative microorganisms.Fermentation...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

An energy-carbon dual-control biohybrid: integrating photosynthetic microorganisms with patterned perovskite solar cells for concurrent electricity and biopolymer production.

Bioresource technology·2026
Same author

Morphological Control of TiO<sub>2</sub> Supports for Enhanced Pt-Catalyzed Methylcyclohexane Dehydrogenation.

Small science·2026
Same author

Sustainable polyolefin upcycling using liquid organic hydrogen carrier based hydrogen delivery and hydrocracking.

Nature communications·2025
Same author

Improving the Economics of Sustainable Aviation Fuels: System-Level Analyses and Perspective.

ChemSusChem·2025
Same author

Ecofriendly Upcycling of Poly(vinyl chloride) Waste Plastics into Precious Metal Adsorbents.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2025
Same author

Python-Based Algorithm for Calculating Physical Properties of Aqueous Mixtures Composed of Substances Not Available in Databases.

ACS omega·2025
Same journal

Biobased Lignin as a Sustainable Flame Retardant Additive for Recycled Polyester Fabrics.

ACS omega·2026
Same journal

Practical Microwave-Assisted Recirculated Flow Esterification at Atmospheric Pressure.

ACS omega·2026
Same journal

Effects of Fiber Inclusion on Pervious Concrete: A Multiscale Experimental and Imaging Study.

ACS omega·2026
Same journal

Investigation on the Influence Mechanisms and Graded Monitoring of CO<sub>2</sub> Leakage on Surface Water Environmental Indicators.

ACS omega·2026
Same journal

The N‑Glycoproteomic Landscape of the Lung in Monocrotaline-Induced Pulmonary Arterial Hypertension.

ACS omega·2026
Same journal

Immobilized Copper-Substituted Keggin POM on Graphene Oxide for Highly Selective Heterogeneous Catalysis of Starch Hydrolysis.

ACS omega·2026
See all related articles
  1. Home
  3. Recovery Yield And Defective Product Rate In A Bio-based Amino Acid Crystallization Process.
  1. Home
  3. Recovery Yield And Defective Product Rate In A Bio-based Amino Acid Crystallization Process.

Related Experiment Video

Improving the Success Rate of Protein Crystallization by Random Microseed Matrix Screening
12:24

Improving the Success Rate of Protein Crystallization by Random Microseed Matrix Screening

Published on: August 31, 2013

Recovery Yield and Defective Product Rate in a Bio-Based Amino Acid Crystallization Process.

Jin Tae Hong1, Jin Joo Oh1, Jung Won Park1

  • 1CJ BIO Research Institute, CJ CheilJedang, Suwon-Si, Gyeonggi-do 16495, Republic of Korea.

ACS Omega
|June 22, 2026

View abstract on PubMed

Summary
This summary is machine-generated.

Predicting crystal purity and recovery yield in biobased crystallization processes is now easier. This study introduces a new method using an effective distribution model for amino acids like l-alanine (ALA) and l-histidine (HIS).

More Related Videos

Optimizing the Growth of Endothiapepsin Crystals for Serial Crystallography Experiments
09:52

Optimizing the Growth of Endothiapepsin Crystals for Serial Crystallography Experiments

Published on: February 4, 2021

ABCG5/G8 Crystallization in a Lipidic Bicelle Environment for X-Ray Crystallography
06:47

ABCG5/G8 Crystallization in a Lipidic Bicelle Environment for X-Ray Crystallography

Published on: August 25, 2023

Related Experiment Videos

Improving the Success Rate of Protein Crystallization by Random Microseed Matrix Screening
12:24

Improving the Success Rate of Protein Crystallization by Random Microseed Matrix Screening

Published on: August 31, 2013

Optimizing the Growth of Endothiapepsin Crystals for Serial Crystallography Experiments
09:52

Optimizing the Growth of Endothiapepsin Crystals for Serial Crystallography Experiments

Published on: February 4, 2021

ABCG5/G8 Crystallization in a Lipidic Bicelle Environment for X-Ray Crystallography
06:47

ABCG5/G8 Crystallization in a Lipidic Bicelle Environment for X-Ray Crystallography

Published on: August 25, 2023

Area of Science:

  • Biochemical Engineering
  • Separation Processes
  • Crystallization Technology

Background:

  • Biobased crystallization processes often lack experimental data, hindering accurate prediction of crystal purity and recovery yield.
  • Challenges in designing and evaluating these processes stem from limited predictive models for amino acid crystallization.

Purpose of the Study:

  • To develop a predictive method for crystal purity and recovery yield in biobased evaporative crystallization of amino acids.
  • To establish a model describing the relationship between liquid and crystal purity.
  • To predict defective product disturbances in the crystallization process.

Main Methods:

  • An effective distribution model, using an empirically modified logistic curve, was developed to link liquid and crystal purity.
  • Mass balance principles were integrated with the effective distribution model to predict crystal purity based on feed purity and recovery yield.
  • A statistical approach was employed to forecast defective product rates.

    • Main Results:

    • The study successfully predicted crystal purity and recovery yield for l-alanine (ALA), l-histidine monohydrochloride monohydrate (HIS), and l-citrulline (CIT).
    • l-Histidine (HIS) demonstrated a higher propensity for achieving superior crystal purity compared to l-alanine (ALA).
    • Defective product rates decreased with increasing target crystal purity and decreasing minimum specification purity across all amino acids studied.

    Conclusions:

    • The developed method provides a robust framework for predicting performance in biobased amino acid crystallization.
    • Recovery yield is identified as a critical metric for the design and evaluation of downstream biobased processes.
    • The findings facilitate more accurate process design and optimization, reducing prediction inaccuracies due to data scarcity.