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Related Concept Videos

Neuronal Communication01:28

Neuronal Communication

Neurons, the fundamental units of the brain and nervous system, communicate through complex electrochemical signals that underpin all cognitive and bodily functions. This communication is primarily facilitated by a process involving the generation and propagation of an action potential along the axon of the neuron. When the internal electrical charge of a neuron surpasses a certain threshold, an action potential is triggered. This rapid change in voltage travels swiftly along the axon to the...
Constitutive and Regulated Gene Expression01:27

Constitutive and Regulated Gene Expression

Gene expression in prokaryotes is governed by constitutive and regulated systems, allowing cells to balance the production of essential proteins with adaptive responses to environmental changes.Constitutive Gene ExpressionConstitutive, or housekeeping, genes are continuously expressed as they encode proteins vital for fundamental cellular processes. These include enzymes for glycolysis, ribosomal components for protein synthesis, and proteins involved in DNA replication. Their constant...
Neural Regulation01:37

Neural Regulation

Digestion begins with a cephalic phase that prepares the digestive system to receive food. When our brain processes visual or olfactory information about food, it triggers impulses in the cranial nerves innervating the salivary glands and stomach to prepare for food.
Regulation of Expression Occurs at Multiple Steps02:24

Regulation of Expression Occurs at Multiple Steps

Gene expression can be regulated at almost every step from gene to protein. Transcription is the step that is most commonly regulated. This involves the binding of proteins to short regulatory sequences on the DNA. This association can either promote or inhibit the transcription of a gene associated with the respective sequence.
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Regulation of Expression Occurs at Multiple Steps02:24

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Transcription results in the generation of precursor (pre-mRNA) that consists of both exons and introns, which needs further processing before being translated to a...
Regulation of Expression at Multiple Steps01:23

Regulation of Expression at Multiple Steps

The gene expression in cells is regulated at different stages: (i) transcription, (ii) RNA processing, (iii) RNA localization, and (iv) translation. Transcriptional regulation is mediated by regulatory proteins such as transcription factors, activators, or repressors—these control gene expression by initiating or inhibiting the transcription of genes. Once a precursor or pre-mRNA is produced, it undergoes post-transcriptional modification, including 5' capping, splicing, and the addition of a...

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Related Experiment Video

Updated: Jun 23, 2026

Induction of an Isoelectric Brain State to Investigate the Impact of Endogenous Synaptic Activity on Neuronal Excitability In Vivo
10:19

Induction of an Isoelectric Brain State to Investigate the Impact of Endogenous Synaptic Activity on Neuronal Excitability In Vivo

Published on: March 31, 2016

A Neuronal Gene Expression Program Underlying Sustained Network Activity.

J Wren Kim, Rebecca Eliscu, Adeline J H Yong

    Biorxiv : the Preprint Server for Biology
    |June 22, 2026
    PubMed
    Summary
    This summary is machine-generated.

    Researchers discovered a new gene expression program in active neurons that helps maintain stable brain network activity. This program, identified using calcium-dependent ribosome tagging (CalTRAP-seq), differs from immediate early gene responses and involves splicing regulation.

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    Inducing Long-Term Plasticity of Intrinsic Neuronal Excitability in Neurons of the Dorsal Lateral Geniculate Nucleus
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    Inducing Long-Term Plasticity of Intrinsic Neuronal Excitability in Neurons of the Dorsal Lateral Geniculate Nucleus

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    Related Experiment Videos

    Last Updated: Jun 23, 2026

    Induction of an Isoelectric Brain State to Investigate the Impact of Endogenous Synaptic Activity on Neuronal Excitability In Vivo
    10:19

    Induction of an Isoelectric Brain State to Investigate the Impact of Endogenous Synaptic Activity on Neuronal Excitability In Vivo

    Published on: March 31, 2016

    Time-dependent Increase in the Network Response to the Stimulation of Neuronal Cell Cultures on Micro-electrode Arrays
    10:45

    Time-dependent Increase in the Network Response to the Stimulation of Neuronal Cell Cultures on Micro-electrode Arrays

    Published on: May 29, 2017

    Inducing Long-Term Plasticity of Intrinsic Neuronal Excitability in Neurons of the Dorsal Lateral Geniculate Nucleus
    05:01

    Inducing Long-Term Plasticity of Intrinsic Neuronal Excitability in Neurons of the Dorsal Lateral Geniculate Nucleus

    Published on: September 20, 2024

    Area of Science:

    • Neuroscience
    • Molecular Biology
    • Genetics

    Background:

    • Neurons operate in networks requiring continuous firing adjustments for stability.
    • The gene expression programs supporting sustained network activity are not well understood.
    • Long-lasting molecular changes are necessary for these adjustments.

    Purpose of the Study:

    • To identify and characterize the gene expression programs active neurons use to maintain stable network function.
    • To explore the molecular mechanisms underlying sustained neuronal network activity.

    Main Methods:

    • Developed CalTRAP-seq (calcium-dependent ribosome tagging sequencing) to profile gene expression in active neurons.
    • Applied CalTRAP-seq to primary neurons exhibiting synchronous network bursting.
    • Investigated nuclear speckle formation and its role in splicing regulation.

    Main Results:

    • Identified a gene expression program distinct from immediate early genes, enriched for neuronal excitability regulators.
    • Observed widespread alternative splicing of synaptic genes in active neurons.
    • Found increased nuclear speckle formation in active neurons, which are implicated in splicing regulation.
    • Demonstrated that disrupting nuclear speckles impairs synchronous burst dynamics.

    Conclusions:

    • Sustained network activity involves a unique gene expression program complementary to stimulus-responsive pathways.
    • Nuclear speckles and alternative splicing play a crucial role in regulating gene expression for stable network function.
    • This study provides insights into how neurons coordinate molecular changes to support stable network activity.