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Updated: Jun 23, 2026

Visualizing Cellular Gibberellin Levels Using the nlsGPS1 Förster Resonance Energy Transfer (FRET) Biosensor
Published on: January 12, 2019
Integrated transcriptomic and proteomic analysis reveals the regulatory role of exogenous gibberellin in sugarcane
Rongfa Chen1, Zongmeng Wu1, Zhenguang Lai1
1Sugarcane Research Institute, Guangxi Academy of Agricultural Sciences/Guangxi Key Laboratory of Sugarcane Biotechnology and Genetic Improvement, Ministry of Agriculture and Rural Affairs/Guangxi Key Laboratory of Sugarcane Genetic Improvement/State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, Nanning, China.
Introduction:
Sugarcane cultivation is a vital component of the agricultural economy in southern China. Investigating internode development in sugarcane is crucial for optimizing cultivation management practices and improving cane yield.
Methods:
In this study, transcriptome and proteome sequencing were performed on internode tissues of sugarcane cultivar Guitang 42 at 0, 6, and 12 days post-treatment, aiming to identify key molecular components and elucidate biological pathways through which exogenous gibberellic acid (GA3) regulates internode maturation.
Results:
Accordingly, GA3 predominantly promoted internodal elongation, rather than nodal expansion. Following transcriptome and proteome sequencing, 3D principal component analysis (PCA) based on both datasets revealed a clear separation between the GA3-treated (GA) and control (CK) groups. The comparison of GA_6d vs. CK_0d identified the largest number of differentially expressed genes (DEGs, 34,541), followed by CK_12d vs. CK_0d (27,898) and GA_12d vs. CK_0d (22,709). Similarly, the pairwise comparison between GA_6d and CK_0d yielded the highest number of differentially expressed proteins (DEPs, 363). KEGG enrichment analysis based on DEGs, DEPs and their intersection revealed that GA3 treatment up-regulated the phenylpropanoid biosynthesis and phenylalanine metabolism pathways, thereby promoting lignin biosynthesis. Additionally, PPI analysis revealed high-confidence interactions between two hub proteins (PAL and 4CL). Finally, we elucidated the biosynthetic pathways that produce p-hydroxyphenyl lignin, guaiacyl lignin, and syringyl lignin using L-phenylalanine as the substrate.
Discussion:
The results presented herein provide new insights into sugarcane internode maturation.
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