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Related Experiment Video

Updated: Jun 24, 2026

Bioprinting Cellularized Constructs Using a Tissue-specific Hydrogel Bioink
08:34

Bioprinting Cellularized Constructs Using a Tissue-specific Hydrogel Bioink

Published on: April 21, 2016

Biological Standardization of Human ASC-Based Biofabrication for Reproducible Macroscale Tissue Constructs.

Aline Pimentel1, Larissa Resende Vieira2, Bianca Montenegro1

  • 1Laboratory of Eukaryotic Cell Biology (Labio), National Institute of Metrology, Quality and Technology (INMETRO); Post-graduation Program of Translational Biomedicine (Biotrans), UNIGRANRIO.

Journal of Visualized Experiments : Jove
|June 22, 2026
PubMed

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Summary

This study presents a standardized biofabrication workflow for creating living tissue constructs. This approach enhances reproducibility and shows promise for regenerative medicine applications.

Area of Science:

  • Biomaterials Engineering
  • Tissue Engineering
  • Regenerative Medicine

Background:

  • Lack of standardized protocols hinders biofabrication comparability and clinical translation.
  • Biological standardization is crucial for reproducibility and regulatory alignment in tissue engineering.

Purpose of the Study:

  • To introduce a standardized biofabrication workflow for reproducible tissue construct generation.
  • To demonstrate the efficacy of a 3D-printed Gelatin Methacryloyl (GelMA) scaffold for spheroid bioassembly.

Main Methods:

  • Standardized workflow: monolayer culture of human adipose-derived stem cells (hADSCs), spheroid formation, 3D printing of GelMA scaffolds, and spheroid bioassembly.
  • Quality control metrics integrated for reproducibility.
  • 3D printing of GelMA scaffolds with central pores for spheroid housing.

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Large-Scale, Automated Production of Adipose-Derived Stem Cell Spheroids for 3D Bioprinting
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Large-Scale, Automated Production of Adipose-Derived Stem Cell Spheroids for 3D Bioprinting

Published on: March 31, 2022

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Last Updated: Jun 24, 2026

Bioprinting Cellularized Constructs Using a Tissue-specific Hydrogel Bioink
08:34

Bioprinting Cellularized Constructs Using a Tissue-specific Hydrogel Bioink

Published on: April 21, 2016

Large-Scale, Automated Production of Adipose-Derived Stem Cell Spheroids for 3D Bioprinting
07:40

Large-Scale, Automated Production of Adipose-Derived Stem Cell Spheroids for 3D Bioprinting

Published on: March 31, 2022

Main Results:

  • hADSCs formed uniform spheroids within 24 hours.
  • Spheroids fused continuously within scaffold pores over 72 hours, forming macroscale constructs.
  • 3D-printed scaffold dimensions showed minor, non-significant deviations from the CAD model, ensuring structural integrity.

Conclusions:

  • The standardized workflow enables reproducible biofabrication of living tissue constructs.
  • Spheroid fusion within 3D-printed scaffolds yields versatile constructs suitable for transplantation.
  • This approach has significant implications for advancing clinical applications in regenerative medicine.