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Related Experiment Video

Updated: Jun 24, 2026

Rapid Antimicrobial Susceptibility Testing by Stimulated Raman Scattering Imaging of Deuterium Incorporation in a Single Bacterium

Rapid Antimicrobial Susceptibility Testing by Stimulated Raman Scattering Imaging of Deuterium Incorporation in a Single Bacterium

Published on: February 14, 2022

On-Chip Raman Spectroscopy for Rapid Antimicrobial Susceptibility Testing from Blood Cultures.

Richard Grohs^1,2,3, Franziska Hornung⁴, Marie-Luise Enghardt^1,2,3

¹Leibniz Institute of Photonic Technology, Member of Leibniz Health Technologies, Member of the Leibniz Research Alliance INFECTIONS, Member of the Leibniz Centre for Photonics in Infection Research (LPI), Albert-Einstein-Straße 9, 07745 Jena, Germany.

Analytical Chemistry

|June 22, 2026

Summary

This summary is machine-generated.

The novel RamanBioAssay (RBA) platform offers rapid antimicrobial susceptibility testing (AST) and bacterial identification within hours. This innovative diagnostic tool significantly reduces turnaround time for bloodstream infections compared to traditional methods.

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Published on: May 24, 2024

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Last Updated: Jun 24, 2026

Rapid Antimicrobial Susceptibility Testing by Stimulated Raman Scattering Imaging of Deuterium Incorporation in a Single Bacterium

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Published on: February 14, 2022

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Published on: July 9, 2012

Direct Microbial Identification using An Automated Microbial Identification System to Facilitate the EUCAST RAST Method Without Mass Spectrometry

Direct Microbial Identification using An Automated Microbial Identification System to Facilitate the EUCAST RAST Method Without Mass Spectrometry

Published on: May 24, 2024

Area of Science:

Biomedical Diagnostics
Spectroscopy
Microbiology

Background:

Bloodstream infections cause significant morbidity and mortality.
Current antimicrobial susceptibility testing (AST) methods are often time-consuming or lack phenotypic relevance.
Rapid diagnostics are crucial for effective treatment of bloodstream infections.

Purpose of the Study:

To introduce the RamanBioAssay (RBA) platform for rapid phenotypic AST and bacterial identification.
To evaluate the performance of the RBA platform in terms of speed and accuracy.
To compare RBA results with conventional AST methods.

Main Methods:

Integration of dielectrophoretic on-chip bacterial enrichment with label-free Raman spectroscopy.
Phenotypic AST and bacterial identification performed on positive blood cultures.
Validation using quality control strains and clinical blood culture samples.

Main Results:

RBA platform provided AST results within 3.5 hours, significantly faster than standard methods.
High concordance rates observed with conventional AST for E. coli and S. aureus.
Accurate bacterial identification (family level) achieved with 97.2% accuracy.

Conclusions:

The RBA platform is a promising tool for rapid phenotypic AST and bacterial identification.
It offers clinically actionable results significantly faster than current diagnostic approaches.
RBA has the potential to improve patient outcomes for bloodstream infections.