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Related Experiment Video

Updated: Jun 24, 2026

Live Cell Imaging during Mechanical Stretch
07:42

Live Cell Imaging during Mechanical Stretch

Published on: August 19, 2015

Universal Pressure-Loading Device for Live-Cell Imaging under Sustained Physiological Mechanical Stress.

Wei Yan1, Yixi Zhang1, Lu Yang1

  • 1Department of Biophysics, School of Life Science and Technology, University of Electronic Science and Technology of China.

Journal of Visualized Experiments : Jove
|June 22, 2026
PubMed
Summary

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Researchers developed a hydrostatic pressure device for real-time cell imaging. This tool enables detailed observation of cell responses to mechanical stress, advancing mechanobiology research.

Area of Science:

  • Mechanobiology
  • Cell Biology
  • Biophysics

Background:

  • Conventional cell culture methods often fail to replicate the mechanical forces experienced by cells in vivo.
  • Understanding cellular responses to mechanical stimuli is crucial for various biological processes and disease mechanisms.
  • Existing techniques for applying mechanical stress to cells often lack real-time imaging capabilities.

Purpose of the Study:

  • To describe a novel hydrostatic pressure-loading device for live-cell imaging.
  • To enable real-time observation of cellular behavior under sustained hydrostatic pressure.
  • To provide a versatile platform for mechanobiological studies.

Main Methods:

  • Fabrication of an airtight culture chamber with an optically transparent cover and integrated gas ports.

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Related Experiment Videos

Last Updated: Jun 24, 2026

Live Cell Imaging during Mechanical Stretch
07:42

Live Cell Imaging during Mechanical Stretch

Published on: August 19, 2015

High-resolution Imaging of Nuclear Dynamics in Live Cells under Uniaxial Tensile Strain
09:20

High-resolution Imaging of Nuclear Dynamics in Live Cells under Uniaxial Tensile Strain

Published on: June 2, 2019

  • Utilizing a regulated gas source to maintain adjustable hydrostatic pressure (0-200 kPa).
  • Integration with phase-contrast and fluorescence microscopy for real-time imaging of adherent cells.

    • Main Results:

    • Demonstrated the device's compatibility with standard 3.5 cm cell culture dishes.
    • Successfully recorded pressure-induced, dose-dependent effects on cell morphology, proliferation, and migration.
    • Quantified pressure-triggered calcium (Ca²⁺) signaling dynamics in MDA-MB-231 and HeLa cancer cells using time-lapse fluorescence microscopy.

    Conclusions:

    • The developed hydrostatic pressure-loading device facilitates real-time microscopic observation of cellular responses to mechanical stimulation.
    • This platform overcomes limitations of endpoint assays, offering a universal tool for mechanobiological research.
    • Enables detailed investigation of mechanotransduction pathways and cellular mechanosensing.