A method for quantifying non-esterified cis-9,trans-11-conjugated linoleic acid in finger-prick plasma by...

cis-9,trans-11-Conjugated linoleic acid (c9,t11-CLA) is the major CLA consumed from ruminant-derived foods and exhibits anti-carcinogenic, anti-atherogenic and anti-Alzheimer's disease effects for humans. Determination of the circulating non-esterified c9,t11-CLA helps provide a better understanding of its health benefits, but very few methods are available for this purpose. In this study, a liquid chromatography/electrospray ionization-tandem mass spectrometry-based method was developed and validated for quantifying non-esterified c9,t11-CLA in plasma. The DEMECAL device enabled not only the minimally invasive collection of finger-prick blood, but also an easy and rapid preparation of the plasma sample. The developed method employed the derivatization with 4-(4-dimethylaminophenyl)-1,2,4-triazoline-3,5-dione (DAPTAD), which achieved the complete separation of c9,t11-CLA from its positional and geometric isomers on an octadecylsilyl silica column (resolution, ≥ 1.92), elimination of the negative influence from linoleic acid, and a higher sensitivity (limit of detection, 210 amol) than any methods currently available. In addition, high precision (relative standard deviations in five repetitive measurements, ≤ 6.1%) and accuracy (analytical recovery rates, 99.4-103.7%) were obtained by the isotope-coded derivatization using deuterium-labeled DAPTAD. This method also had a sufficient applicability to real sample analysis; we successfully determined the plasma non-esterified c9,t11-CLA concentrations of Japanese healthy subjects and detected their changes due to the CLA supplementation.