Related Experiment Video
Updated: Jun 24, 2026

Measurement of Ultrafast Vibrational Coherences in Polyatomic Radical Cations with Strong-Field Adiabatic Ionization
Published on: August 6, 2018
Oxidation Products of S-Adenosyl Methionine Probed by Infrared Multiple Photon Dissociation Spectroscopy
Jean-Xavier Bardaud1, Yining Jiang1, Carine Clavaguera1
1CNRS, Université Paris-Saclay, CNRS, Institut de Chimie Physique, Orsay, France.
Abstract:
S-adenosyl methionine (SAM) is a key biomolecule in cellular processes, acting as a primary methyl donor, having different roles in enzymatic reactions and being used as a scavenger of hydroxyl radicals. Due to the role played by SAM and its antioxidant properties, the one electron oxidation products of SAM produced by gamma radiolysis, which can mimic the oxidation effect produced by hydroxyl radical in the oxidative stress process, have been investigated in this study by collision-induced dissociation tandem mass spectrometry (CID-MS2) and infrared multiple photon dissociation spectroscopy (IRMPD). We have revealed the modified sites in the molecule and characterized the 3D structure of the oxo-forms of SAM in the gas phase. Both CID-MS2 and IRMPD experiments, when coupled with quantum chemistry calculations have shown that only the adenine moiety of the molecule is oxidized, protecting the methionine from oxidation.
More Related Videos
10:03Proton Transfer and Protein Conformation Dynamics in Photosensitive Proteins by Time-resolved Step-scan Fourier-transform Infrared Spectroscopy
Published on: June 27, 2014
08:23Analyzing the Photo-oxidation of 2-propanol at Indoor Air Level Concentrations Using Field Asymmetric Ion Mobility Spectrometry
Published on: June 14, 2018
Related Concept Videos
Infrared (IR) Spectroscopy: Overview
Different compounds display unique properties due to their...
IR Spectroscopy: Molecular Vibration Overview
Stretching vibrations are vibrational motions that occur along the bond line, changing the bond length or distance between two bonded atoms. They are further distinguished as symmetric or asymmetric. In symmetric stretching, the...
IR and UV–Vis Spectroscopy of Aldehydes and Ketones
Tandem Mass Spectrometry
¹H NMR: Complex Splitting
Splitting diagrams or splitting tree diagrams are routinely used to depict such complex couplings. While drawing splitting diagrams, the splitting with the larger coupling constant is usually applied first.
UV–Vis Spectroscopy: Molecular Electronic Transitions