Dual-coating strategies for improving the storage stability of bioactive-loaded liposomes

This study evaluated how secondary coating materials modulate the storage stability of liposomes encapsulating Deinococcus radiodurans extracts. Liposomes prepared with soybean-derived phosphatidylcholine were subsequently coated with different cationic materials via electrostatic deposition. Compared with uncoated liposomes, dual-coated systems exhibited distinct changes in particle size distribution and surface charge, indicating successful multilayer formation. Secondary coating increased encapsulation efficiency from 66.5% in primary liposomes to 87.2 and 92.5% in Eco CAT MB- and chitosan-coated liposomes, respectively. After 28 d of storage, chitosan-coated liposomes retained the highest encapsulation efficiency (69.8%) and maintained antioxidant activity more effectively than primary liposomes. These findings demonstrate that coating-layer properties, particularly the polymeric barrier effect of chitosan, play a key role in improving the storage stability of bioactive-loaded liposomes.

The online version contains supplementary material available at 10.1007/s10068-026-02179-9.